引用本文:邓 爽,赵红英,韦 胜,卢立江,李乃梅,梁 莉,黄 瑛,赖立川,赖国建,陈宗波,卢建辉,黄华艺.溶组织内阿米巴5例的粪便鉴定[J].中国临床新医学,2018,11(11):1129-1132.
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溶组织内阿米巴5例的粪便鉴定
邓 爽,赵红英,韦 胜,卢立江,李乃梅,梁 莉,黄 瑛,赖立川,赖国建,陈宗波,卢建辉,黄华艺
530021 南宁,广西壮族自治区人民医院检验科(邓 爽,赵红英,韦 胜,卢立江,李乃梅,梁 莉,黄 瑛,赖立川,赖国建,陈宗波,卢建辉,黄华艺);14263 纽约,布法罗 美国罗斯威尔帕克综合癌症中心肿瘤外科(黄华艺)
摘要:
[摘要] 目的 探讨溶组织内阿米巴的粪便鉴定,并就溶组织内阿米巴包囊及滋养体的镜检要点及常见漏检原因进行讨论。方法 用显微镜法对5例外院漏诊的肠阿米巴病患者粪便标本进行检验,分别用生理盐水涂片法、碘染色和苏木素染色法,在显微镜下进行观察鉴定。结果 溶组织内阿米巴包囊及滋养体在生理盐水涂片、碘染色涂片和苏木素染色涂片中有典型的形态学特点。结论 对于可疑肠阿米巴病患者,应多次及时送标本进行检验,采用几种不同方法进行鉴定,并注意将溶组织内阿米巴与吞噬细胞及迪斯帕内阿米巴相鉴别。
关键词:  肠阿米巴病  溶组织内阿米巴  迪斯帕内阿米巴  吞噬细胞
DOI:10.3969/j.issn.1674-3806.2018.11.17
分类号:R 445
基金项目:
Characterization of Entamoeba histolytica in feces: a report of 5 cases
DENG Shuang, ZHAO Hong-ying, WEI Sheng, et al.
Department of Laboratory Medicine, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning,530021, China
Abstract:
[Abstract] Objective To characterize the Entamoeba histolytica of 5 cases in feces to avoid missing inspection. Methods Five stool samples from the patients with Entamoeba histolytica infection but missed diagnosis in the other hospitals were analyzed using normal saline smear, iodine staining and hematoxyline staining under microscopy. Results The amebic cyst and amebic trophozoite of Entamoeba histolytica displayed unique characteristics by using normal saline smear, iodine staining and hematoxyline staining. Conclusion  For the patients with suspected intestinal amoeba, stool specimens should be sent to the laboratory repeatedly and timely for analysis. The Entamoeba histolytica should be identified by using different methods. Special attentions must be paid to identify the Entamoeba histolytica and distinguish it from phagocytes and Entamoeba dispar.
Key words:  Amebic dysentery  Entamoeba histolytica  Entamoeba dispar  Phagocytes