引用本文:巢潇莺,钟国强,蒋智渊,黎珊珊.人类miRNA-613的靶基因预测及生物信息学分析[J].中国临床新医学,2019,12(4):381-385.
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人类miRNA-613的靶基因预测及生物信息学分析
巢潇莺,钟国强,蒋智渊,黎珊珊
530021 南宁,广西医科大学第一附属医院心血管内科(巢潇莺,钟国强),高血压科(蒋智渊),心电诊断科(黎珊珊)
摘要:
[摘要] 目的 利用生物信息学技术分析及预测人类miRNA-613(hsa-miR-613)的靶基因及其可能参与的生物学过程,为更深入的研究微小RNA与心脏疾病的关系提供基础。方法 通过miRbase数据库和UCSC Genome Browser获取hsa-miR-613的序列及其染色体定位和物种间保守性等基本信息。应用miRDB、TargetScan、miRanda、DIANA-microT对hsa-miR-613进行靶基因预测取交集,合并miRTarbase数据库中已被实验证实的靶基因作为基因集,进行功能注释(GO分析)和pathway信号通路富集分析。结果 hsa-miR-613在物种间高度保守,其靶基因功能富集于血管发育、细胞增殖发育等生物学过程。靶基因存在于细胞各个组分,例如细胞质、细胞器、黏着斑等,其分子功能富集于蛋白结合和转录因子活性的调控等(P<0.05)。信号通路分析提示靶基因的信号通路显著富集于突触囊泡循环、剪接体、PI3K-Akt信号通路、mTOR信号通路、黏着斑等通路中,以及包括先天性心脏病的先天性疾病、心血管系统疾病的相关通路中(P<0.05)。结论 hsa-miR-613预测的靶基因富集于多个生物学过程,与心脏发育过程密切相关,为进一步研究提供了生物学基础。
关键词:  微小RNA-613  人类  靶基因  生物信息学  心脏疾病
DOI:10.3969/j.issn.1674-3806.2019.04.07
分类号:R 318.5
基金项目:国家自然科学基金资助项目(编号:81660054);广西自然科学基金资助项目(编号:2016GXNSFBA380115)
Prediction of homo sapiens-miRNA-613 target gene and its bioinformatics analysis
CHAO Xiao-ying, ZHONG Guo-qiang, JIANG Zhi-yuan, et al.
Department of Cardiovascular Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
Abstract:
[Abstract] Objective To analyze and predict the target genes of homo sapiens miRNA-613(hsa-miR-613) and their possible biological processes by using bioinformatics techniques, and provide a basis for further research on the relationship between microRNA and heart diseases. Methods The basic data of hsa-miR-613 including chromosomal location, base sequence and conservation of species were obtained from miRbase database and UCSC Genome Browser. Target genes of hsa-miR-613 were predicted by miRDB, TargetScan, miRanda and DIANA-microT and then the intersection was performed. The target genes supported by literature and confirmed by experiments from miRTarbase database were combined as a target gene set, which was analyzed with Gene Ontology(GO) analysis and signal pathway analysis. Results The sequence of hsa-miR-613 was highly conserved among virious species, and its target gene functions were enriched in biological processes such as vascular development, cell proliferation and development; target genes existed in various components of the cell such as cytoplasm, organelles, focal adhesion, etc and molecular functions were enriched in protein binding and transcription factor activities and so on(P<0.05). The signal pathway analysis showed that the target genes were enriched in the pathways including synaptic vesicle cycle, spliceosome, PI3K-Akt signaling pathway(PI3K-Akt), mTOR signaling pathway, focal adhesion, and other pathways including congenital diseases and cardiovascular diseases including congenital heart disease(P<0.05). Conclusion The predicted target genes of hsa-miR-613 enrich in multiple biological processes and are related to cardiac development, which provides a biological basis for further research.
Key words:  microRNA-613  Homo sapiens  Target gene  Bioinformatics  Heart disease