引用本文:周亭亭,薛林涛,王世凯,谭卫红,成俊萍,毛献宝,李政达,张小慧,韦娉嫔.人工皱缩对玻璃化冻融囊胚复苏结局的影响[J].中国临床新医学,2019,12(5):522-525.
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人工皱缩对玻璃化冻融囊胚复苏结局的影响
周亭亭,薛林涛,王世凯,谭卫红,成俊萍,毛献宝,李政达,张小慧,韦娉嫔
530021 南宁,广西壮族自治区人民医院生殖医学与遗传中心
摘要:
[摘要] 目的 探讨不同人工皱缩方式对囊胚玻璃化冻融后复苏结局的影响。方法 收集体外受精(IVF)/卵泡浆内单精子注射(ICSI)治疗周期病人冷冻或移植后的246枚囊胚,随机分为对照组(囊胚不进行皱缩,直接玻璃化冻融)、激光皱缩组(囊胚采用激光打孔法使其完全皱缩后行玻璃化冻融)、机械皱缩组(囊胚采用机械挤压法使其完全皱缩后行玻璃化冻融),每组82枚。比较三组间的复苏率和孵出率。结果 激光皱缩组和机械皱缩组囊胚玻璃化冻融后的复苏率和孵出率均高于对照组(P<0.05)。机械皱缩组和激光皱缩组的优质囊胚孵出率均高于对照组且差异有统计学意义(P<0.05)。激光皱缩组优质囊胚复苏率及孵出率均高于机械皱缩组,但差异无统计学意义(P>0.05)。机械皱缩组和激光皱缩组的劣质囊胚复苏率、孵出率均高于对照组(P<0.05),激光皱缩组劣质囊胚孵出率高于机械皱缩组(P<0.05)。结论 人工皱缩去除囊胚腔液能够显著改善囊胚玻璃化冻融复苏结局,而激光法皱缩是最佳的人工皱缩方式。
关键词:  人工皱缩  囊胚  玻璃化冷冻  复苏率  孵出率
DOI:10.3969/j.issn.1674-3806.2019.05.13
分类号:R 715
基金项目:广西卫健委科研课题(编号:Z2015305)
Effects of artificial shrinkage before vitrification on survival rate and hatching efficiency in human blastocysts freeze-thaw
ZHOU Ting-ting, XUE Lin-tao, WANG Shi-kai, et al.
Reproductive Medical and Genetic Center, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
Abstract:
[Abstract] Objective To investigate the effects of different artificial shrinking methods before vitrified cryopreservation on survival rate and hatching efficiency in human blastocysts freeze-thaw. Methods Two hundred and forty-six surplus blastocysts after vitrification or transplantation in IVF/ICSI cycles were divided into control group(with untreated blastocysts), laser drilling group(with artificial shrinking performed by a laser pulse prior to vitrification), and pipetting group(with mechanical extruding the blastocyst prior to vitrification), with 82 cases in each group. The survival rate and hatching rate were compared among the three groups. Results The survival rate and the hatching rate in the laser drilling group and the pipetting group were higher than those in the control group(P<0.05). For good quality blastocysts, the hatching rates in the laser drilling group and the pipetting group were higher than those in the control group(P<0.05), but there were no significant differences in the proportions of the survival rate and the hatching rate between the laser drilling group and the pipetting group(P>0.05). For poor quality blastocysts, the survival rate and the hatching rate of the two artificial shrinking groups were higher than those of the control group(P<0.05), and the hatching rate of the laser drilling group was higher than that of the pipetting group(P<0.05). Conclusion The outcome of blastocysts freeze-thaw can be significantly improved by artificial shrinkage, which is used to help blastocoele collapse prior to blastocyst vitrification. Laser pulse artificial shrinkage is demonstrated to be a more effective method for blastocyst vitrification.
Key words:  Artificial shrinkage  Blastocyst  Vitrification  Survival rate  Hatching rate