引用本文:蓝倩倩,陈丽妃,黄 慧,沈朝兰,陈 琦.共聚焦显微镜对感染性角膜炎病原学的诊断价值[J].中国临床新医学,2019,12(6):626-629.
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共聚焦显微镜对感染性角膜炎病原学的诊断价值
蓝倩倩,陈丽妃,黄 慧,沈朝兰,陈 琦
530021 南宁,广西壮族自治区人民医院眼科
摘要:
[摘要] 目的 评价共聚焦显微镜(CM)对细菌、真菌、单纯疱疹病毒、棘阿米巴4种感染性角膜炎的病原体的鉴别诊断能力。方法 选择该院2015-01~2018-09临床确诊为4种感染性角膜炎患者,采取盲法利用CM进行病原学的鉴别诊断,并统计其诊断效能。结果 纳入临床确诊的感染性角膜炎共151例,其中细菌性角膜炎43例,真菌性角膜炎56例,单疱病毒性角膜炎49例,棘阿米巴性角膜炎3例。CM对细菌性角膜炎、真菌性角膜炎、单纯疱疹病毒性角膜炎、棘阿米巴性角膜炎病原学诊断的灵敏度依次为76.74%、91.07%、77.56%、100.00%,特异度依次为85.19%、97.89%、92.16%、100.00%,Kappa值依次为0.59、0.90、0.71、1.00。结论 CM对感染性角膜炎的病原体有较好的鉴别诊断能力。
关键词:  共聚焦显微镜  感染性角膜炎  诊断效能
DOI:10.3969/j.issn.1674-3806.2019.06.12
分类号:R 772.21
基金项目:广西卫健委科研课题(编号:Z2016623)
Diagnostic efficiency of confocal microscopy in aetiology of infectious keratitis
LAN Qian-qian, CHEN Li-fei, HUANG Hui, et al.
Department of Ophthalmology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
Abstract:
[Abstract] Objective To evaluate the diagnostic efficiency ability of confocal microscopy(CM) for pathogens of four kinds of infectious keratitis caused by bacteria, fungi, herpes simplex virus and acanthamoeba. Methods We selected the patients diagnosed with the four kinds of infectious keratitis in our hospital. CM was performed to differentially diagnose the pathogens by blind method to analyse the diagnostic efficiency. Results One hundred and fifty-one cases were diagnosed with infectious keratitis, including 43 cases of bacterial keratitis, 56 cases of fungal keratitis, 49 casese of herpes simplex virus keratitis and 3 cases of acanthamoeba keratitis. The sensitivities of CM in diagnosis of bacterial keratitis, fungal keratitis, herpes simplex virus keratitis and acanthamoeba keratitis were 76.74%, 91.07%, 77.56% and 100.00% respectively, and the specificities of the four infections were 85.19%, 97.89%, 92.16% and 100.00% respectively. The Kappa values of the four infections were 0.59, 0.90, 0.71 and 1.00 respectively. Conclusion CM has high efficiency in differential diagnosis of pathogens of infectious keratitis.
Key words:  Confocal microscopy(CM)  Infectious keratitis  Diagnostic efficiency