引用本文:武崇文,杨喜永,赵小燕,郑锡铭.类风湿关节炎患者外周血miR-203和MMP-3表达及其临床意义[J].中国临床新医学,2019,12(9):1011-1014.
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类风湿关节炎患者外周血miR-203和MMP-3表达及其临床意义
武崇文,杨喜永,赵小燕,郑锡铭
463000 河南,驻马店市中心医院检验科
摘要:
[摘要] 目的 探讨微小RNA203(miR-203)和基质金属蛋白酶3(MMP-3)在类风湿关节炎(RA)患者外周血中的表达及其临床意义。方法 选取2016-11~2017-05在该院住院诊断为RA患者60例作为RA组,同期健康体检者30名作为健康对照组,QRT-PCR检测miR-203表达水平,ELISA法检测MMP-3表达水平。ROC曲线分析评价其对RA诊断灵敏度及特异度。结果 RA组miR-203和MMP-3表达均高于对照组,其在活动期升高更明显。相关性分析显示两者存在显著正相关关系(r=0.700,P<0.01);miR-203、MMP-3与类风湿因子(RF)、抗环瓜氨酸抗体(ACCP)、血沉(ESR)、C-反应蛋白(CRP)及28处关节疾病活动度评分(DAS28)存在正相关(r=0.639、0.474、0.528、0.518、0.765、0.816、0.568、0.520、0.613、0.594,P<0.01)。ROC曲线分析表明血浆miR-203及MMP-3对RA具有较高的诊断灵敏度和特异度,联合检测能提高对RA的临床诊断能力。结论 miR-203及MMP-3在RA患者外周血中存在差异表达,并具有较强临床诊断能力,有望成为新的临床诊断指标。
关键词:  微小RNA203  基质金属蛋白酶3  类风湿关节炎
DOI:10.3969/j.issn.1674-3806.2019.09.21
分类号:R 593.24
基金项目:
Expressions and clinical significances of miR-203 and MMP-3 in patients with rheumatoid arthritis
WU Chong-wen, YANG Xi-yong, ZHAO Xiao-yan, et al.
Department of Laboratory, Zhumadian Central Hospital, Henan 463000, China
Abstract:
[Abstract] Objective To investigate the expressions of microRNA-203(miR-203) and matrix metalloproteinase-3(MMP-3) in the peripheral blood of the patients with rheumatoid arthritis(RA) and their clinical significances. Methods Sixty RA patients in our hospital were selected as the RA group, and 30 persons who took physical examination as the healthy control group. The expression of miR-203 was detected by quanticative real-time polyinerase chain reaction(QRT-PCR) and the expression of MMP-3 by enzyme-cinked immunosorbent assay(ELISA). ROC curve analysis was performed to evaluate the diagnostic sensitivity and specificity of RA. Results The expressions of miR-203 and MMP-3 in the RA group were higher than those in the control group, and the expressions increased significantly in the active phase, and there was a significant positive correlation between them(r=0.700, P<0.01). Correlation analysis showed that there were positive correlations between miR-203, MMP-3 and RF, ACCP, ESR, CRP and DAS28(r=0.639, 0.474, 0.528, 0.518, 0.765, 0.816, 0.568, 0.520, 0.613, 0.594, P<0.01). ROC curve analysis showed that plasma miR-203 and MMP-3 had high diagnostic sensitivity and specificity for RA, and the combined detection improved the clinical diagnostic ability of the disease. Conclusion The differential expressions of miR-203 and MMP-3 in the peripheral blood of the patients with RA have strong clinical diagnostic efficacy, and miR-203 and MMP-3 are expected to become a new clinical diagnostic indicators.
Key words:  microRNA-203(miR-203)  Matrix metalloproteinase-3(MMP-3)  Rheumatoid arthritis(RA)