引用本文:李美芳,沈伟强.FeS-PEG载药纳米颗粒对肿瘤细胞杀伤作用的研究[J].中国临床新医学,2019,12(11):1221-1225.
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FeS-PEG载药纳米颗粒对肿瘤细胞杀伤作用的研究
李美芳,沈伟强
215400 江苏,太仓市第一人民医院病案统计室(李美芳),信息中心(沈伟强)
摘要:
[摘要] 目的 探讨具有良好生物相容性的多功能纳米材料FeS-PEG的载药性能,以及其在载带抗肿瘤小分子药物盐酸阿霉素(DOX)后DOX对肿瘤细胞4T1细胞增殖、入胞、凋亡的影响。方法 采用高温合成法合成纳米材料FeS,经层层交联修饰FeS得到FeS-PEG;分析FeS-PEG的细胞毒性、载药性能并进行载药入胞和细胞凋亡检测。结果 FeS-PEG纳米材料对抗肿瘤药物DOX的载药率为134%,载药后在pH=7.4的环境下释药7.13%,pH=6释药10.94%,pH=5释药24.53%。并且FeS-PEG载药后可将DOX药物滞留于细胞质中;游离DOX培养细胞促进细胞凋亡比例为(5.1±0.72)%,而FeS-PEG载药后可促进细胞凋亡比例(31.28±2.28)%。结论 FeS-PEG纳米材料可载带小分子药物,改变小分子药物在细胞内的停留部位,将小分子药物滞留于细胞质中,另外,该纳米材料载带药物后可明显促进细胞凋亡,从而达到增强药物对肿瘤细胞的持久杀伤作用,在药物载带输送方面具有很大应用前景。
关键词:  纳米材料  载药  药物入胞  细胞凋亡
DOI:10.3969/j.issn.1674-3806.2019.11.19
分类号:R 73-3
基金项目:
Study on pegylated FeS nanoparticles as carrier for drug delivery and the killing effect of FeS-PEG loaded nanoparticles on tumor cells
LI Mei-fang, SHEN Wei-qiang
Medical Records and Statistics Room, the First People′s Hospital of Taicang City, Jiangsu 215400, China
Abstract:
[Abstract] Objective To explore the drug loading properties of multi-functional nanometer material pegylated FeS(FeS-PEG) nanoparticles with good biocompatibility, and the effect of doxorubicin(DOX) on the proliferation, endocytosis and apoptosis of tumor cell 4T1 after anti-tumor drug DOX was carried by FeS-PEG. Methods Nanostructured FeS was synthesized by high temperature synthesis, and FeS-PEG was obtained by crosslinking modification of FeS layer by layer. The cytotoxicity and drug loading properties of FeS-PEG were analyzed, and the endocytosis of the loaded drugs and apoptosis were detected. Results The drug loading rate of the anti-tumor drug DOX loaded by FeS-PEG nanomaterials was 134%. The loaded drugs were released by 7.13% if pH = 7.4, by 10.94% if pH=6, by 24.53% if pH=5. The drugs loaded by FeS-PEG could retain DOX in the cytoplasm. The percentage of apoptosis promoted by free DOX culture was (5.1±0.72)%, while that by the drugs loaded with FeS-PEG was (31.28±2.28)%. Conclusion FeS-PEG nanomaterials can carry small molecule drugs, change the retention site of small molecule drugs in cells, and retain small molecule drugs in cytoplasm. In addition, the nanomaterials can obviously promote cell apoptosis after drug loading, so as to enhance the long-term killing effect of drugs on tumor cells, which has a great application prospect in drug delivery.
Key words:  Nanometer materials  Drug loading  Drug endocytosis  Apoptosis