| 摘要: |
| ［摘要］ 目的 探讨红霉素对聚甲基丙烯酸甲酯（PMMA）磨损颗粒刺激下MC3T3-E1细胞成骨作用的影响及其潜在机制。方法 对MC3T3-E1细胞予以不同浓度（0.0 μg/ml、0.2 μg/ml、1.0 μg/ml、5.0 μg/ml和10.0 μg/ml）的红霉素进行预处理，后加入PMMA磨损颗粒进行干预刺激，72 h后对MC3T3-E1细胞的增殖活性及成骨活性进行检测。通过实时定量聚合酶链反应（RT-PCR）法检测MC3T3-E1细胞RUNT相关转录因子2（Runx2)、成骨相关转录因子Osterix、骨钙素（OCN）和p65的表达水平。结果 与空白对照组相比，经PMMA处理组的细胞碱性磷酸酶浓度均显著降低（P<0.05）。经红霉素干预后，细胞碱性磷酸酶浓度升高，呈剂量依赖性。与空白对照组相比，PMMA组Runx2、Osterix、OCN的表达水平均显著降低(P<0.05)，而p65表达水平显著增高(P<0.05)。经0.2 μg/ml、1.0 μg/ml、5.0 μg/ml和10.0 μg/ml红霉素处理后，MC3T3-E1细胞p65表达水平较PMMA组显著降低(P<0.05)。经1.0 μg/ml、5.0 μg/ml和10.0 μg/ml红霉素处理后，MC3T3-E1细胞Runx2、OCN表达水平较PMMA组显著上升(P<0.05)。经5.0 μg/ml和10.0 μg/ml红霉素处理后，MC3T3-E1细胞Osterix表达水平较PMMA组显著上升(P<0.05)。红霉素干预对Runx2、Osterix、OCN和p65表达水平的恢复均呈剂量依赖性。结论 红霉素可有效改善PMMA磨损颗粒对MC3T3-E1细胞成骨活性的抑制作用，而这可能是通过下调NF-κB信号通路实现的。 |
| 关键词: 红霉素 聚甲基丙烯酸甲酯 无菌性松动 磨损颗粒 成骨细胞 |
| DOI：10.3969/j.issn.1674-3806.2021.08.09 |
| 分类号:R 684 |
| 基金项目:国家自然科学基金资助项目（编号：81560364，81760405，81760395，82060408） |
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| Effect of erythromycin on osteogenesis of MC3T3-E1 cells stimulated by polymethyl methacrylate wear particles |
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LIU Zi-ge, ZHANG Chen, SONG Guo-rui, et al.
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Xiamen University School of Medicine, Fujian 361100, China
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| Abstract: |
| ［Abstract］ Objective To explore the effect of erythromycin on the osteogenesis of MC3T3-E1 cells stimulated by polymethyl methacrylate(PMMA) wear particles and its potential mechanisms. Methods MC3T3-E1 cells were pretreated with erythromycin at different concentrations(0.0 μg/ml, 0.2 μg/ml, 1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), and then PMMA wear particles were added for intervention and stimulation. After 72 hours, the proliferation activity and osteogenic activity of MC3T3-E1 cells were detected. Real-time quantitative polymerase chain reaction(RT-PCR) was used to detect the expression levels of RUNT related transcription factor 2(Runx2), osteogenesis-related transcription factor Osterix, osteocalcin(OCN) and p65 in MC3T3-E1 cells. Results Compared with that of the blank control group, the alkaline phosphatase concentration of the PMMA-treated group was significantly decreased(P<0.05). After the intervention of erythromycin, the intracellular alkaline phosphatase concentration increased in a dose-dependent manner. Compared with those in the blank control group, the expression levels of Runx2, Osterix and OCN in the PMMA group were significantly decreased(P<0.05), while the expression level of p65 in the PMMA group was significantly increased(P<0.05). After treatment with erythromycin at different concentrations(0.2 μg/ml, 1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), the p65 expression level of MC3T3-E1 cells was significantly lower than that of PMMA group(P<0.05). After treatment with erythromycin at different concentrations(1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), the expression levels of Runx2 and OCN in MC3T3-E1 cells were significantly higher than those in the PMMA group(P<0.05). After treatment with erythromycin at different concentrations(5.0 μg/ml and 10.0 μg/ml), the expression level of Osterix in MC3T3-E1 cells was significantly higher than that in the PMMA group(P<0.05). The recoveries of Runx2, Osterix, OCN and p65 expression levels intervened by erythromycin were dose-dependent. Conclusion Erythromycin can effectively improve the inhibitory effect of PMMA wear particles on the osteogenic activity of MC3T3-E1 cells, which may be achieved by down-regulating the NF-κB signaling pathway. |
| Key words: Erythromycin Polymethyl methacrylate(PMMA) Aseptic loosening Wear particles Osteoblasts |
