| 摘要: |
| ［摘要］ 目的 探讨淋巴上皮瘤样癌(LELC)临床病理特征和程序性死亡配体1(PD-L1)的表达情况。方法 收集2010年1月至2021年5月暨南大学附属第一医院临床病理科确诊的15例LELC的临床资料。采用免疫组织化学染色法检测PD-L1及广谱细胞角蛋白（pan-CK）、P40、白细胞共同抗原(LCA)、突触素(Syn)和Ki-67。采用原位杂交法检测肿瘤组织中Epstein-Barr(EB)病毒编码的小RNA（EBER）。结果 15例LELC患者中，男4例，女11例，年龄28～81岁，中位年龄59岁。病变部位见于肺部10例，涎腺3例，肾盂1例，鼻腔1例。光学显微镜下，癌细胞呈巢状、弥漫片状或条索状排列；细胞圆形、多角形或不规则，胞质红染，界不清，未分化或低分化状；细胞核深染或呈空泡状，可见明显的核仁及核分裂像；间质伴丰富的淋巴细胞、浆细胞浸润。免疫组织化学染色结果显示pan-CK、P40均为阳性，间质淋巴细胞LCA阳性；14例样本Ki-67增殖指数≥40%。10例样本显示PD-L1膜阳性表达。14例样本EBER均为阳性。结论 LELC是较为罕见的恶性肿瘤，多见于肺部，大部分病变与EB病毒感染相关。大多数病例中肿瘤细胞高表达PD-L1，提示免疫治疗可能获益。 |
| 关键词: 淋巴上皮瘤样癌 程序性死亡配体1 EB病毒 病理特征 |
| DOI：10.3969/j.issn.1674-3806.2021.12.04 |
| 分类号:R 365 |
| 基金项目:国家自然科学基金项目（编号：81572606） |
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| Analysis of the clinicopathological feature and expression of PD-L1 in lymphoepithelioma-like carcinoma |
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ZHOU Ren-yu, ZHONG Li-juan, PENG Li-lin, et al.
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Department of Clinical Pathology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China
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| Abstract: |
| ［Abstract］ Objective To explore the clinicopathological feature and the expression of programmed death-ligand 1(PD-L1) in lymphoepithelioma-like carcinoma(LELC). Methods The clinical data of 15 cases of LELC diagnosed in Department of Clinical Pathology, the First Affiliated Hospital of Jinan University from January 2010 to May 2021 were collected. Immunohistochemical staining method was used to detect PD-L1, pan-cytokeratin(pan-CK), P40, leukocyte common antigen(LCA), synaptophysin(Syn) and Ki-67. In situ hybridization method was used to detect Epstein-Barr(EB) virus-encoded small RNA(EBER) in the tumor tissues. Results Among the 15 LELC patients, there were 4 males and 11 females, aged from 28 to 81 years, with a median age of 59 years. The lesions were found in the lungs in 10 cases, in the salivary glands in 3 cases, in the renal pelvis in 1 case, and in the nasal cavity in 1 case. Under an optical microscope, the cancer cells were arranged in nests, diffuse sheets or strips; the cells were round, polygonal or irregular; the cytoplasm was red-stained, and the borders were unclear, undifferentiated or poorly differentiated; the nuclei were deeply stained or presented vaculating, with obvious nucleoli and mitotic figures; there were abundant lymphocytes and plasma cell infiltration in the stroma. The results of immunohistochemical staining showed that pan-CK and P40 were positive, and interstitial lymphocytes were positive for LCA; Ki-67 proliferation index ≥40% in 14 samples. Ten samples showed positive expression of PD-L1 membrane. Fourteen samples were positive for EBER. Conclusion LELC is a relatively rare malignant tumor and is more common in the lungs. Most of the lesions are related to EB virus infection. In most cases, tumor cells highly express PD-L1, suggesting a possible benefit of immunotherapy. |
| Key words: Lymphoepithelioma-like carcinoma(LELC) Programmed death-ligand 1(PD-L1) Epstein-Barr(EB) virus Pathological feature |
