引用本文:周玉麟,刘 妍,夏 勇,郭旭光.微滴式数字聚合酶链反应在病原体检测中的研究进展[J].中国临床新医学,2022,15(10):914-920.
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微滴式数字聚合酶链反应在病原体检测中的研究进展
周玉麟,刘 妍,夏 勇,郭旭光
510140 广东,广州医科大学附属第三医院检验科
摘要:
[摘要] 聚合酶链反应是一种分子生物学技术,用于使某些脱氧核糖核酸(DNA)片段扩增,它是一种常见且不可或缺的技术,已应用于许多领域,特别是在临床实验室中。第三代聚合酶链反应,微滴式数字聚合酶链反应(ddPCR),是常规聚合酶链反应方法的生物技术改进,可用于直接定量和克隆扩增DNA。ddPCR现在广泛用于低丰度核酸检测,可用于感染性疾病的诊断。该文介绍了ddPCR检测手段的发展及原理,总结了其在病毒性疾病、细菌性疾病和真菌性疾病临床诊断中的潜在优势:ddPCR对低丰度病原体的检测更灵敏、准确、可重复,可能在未来临床应用中是比定量聚合酶链反应更好的选择。
关键词:  微滴式数字聚合酶链反应  病原体  研究进展
DOI:10.3969/j.issn.1674-3806.2022.10.05
分类号:R 446
基金项目:
Research progress of droplet digital PCR in pathogen detection
ZHOU Yu-lin, LIU Yan, XIA Yong, et al.
Department of Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangdong 510140, China
Abstract:
[Abstract] Polymerase chain reaction(PCR) is a molecular biology technique used to amplify certain fragments of deoxyribonucleic acid(DNA). It is a common and indispensable technique that has been used in many fields, especially in clinical laboratories. Droplet digital polymerase chain reaction(ddPCR) is a third-generation PCR, which is a biotechnology obtained by improving the conventional PCR method, and can be used to directly quantify and clonally amplify DNA.Droplet digital PCR is now widely used for low-abundance nucleic acid detection, which can be used for the diagnosis of infectious diseases. This paper introduces the development and principle of ddPCR detection means, summarizes its potential advantages in the clinical diagnosis of viral diseases, bacterial diseases and fungal diseases, and draws the conclusion that ddPCR is more sensitive and accurate and repeatable for the detection of low-abundance pathogens, and may be a better choice than quantitative PCR in future clinical applications.
Key words:  Droplet digital polymerase chain reaction(ddPCR)  Pathogen  Research progress