| 摘要: |
| ［摘要］ 目的 探究肽核酸-荧光原位杂交（PNA-FISH）、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）快速鉴定白念珠菌的临床应用价值。方法 采用经VITEK 2 Compact全自动细菌鉴定仪YST卡鉴定白念珠菌100株，模拟血培养阳性标本。对报阳的标本直接进行PNA-FISH鉴定，再转种培养出单个新鲜菌落，完成MALDI-TOF MS鉴定。记录两种方法鉴定白念珠菌结果和鉴定所需时间，计算其与VITEK 2 Compact全自动细菌鉴定方法的符合率。结果 100株白念珠菌模拟血培养样本，PNA-FISH鉴定结果与VITEK 2 Compact全自动细菌鉴定方法的符合率为98%，平均鉴定时间为（2.5±0.5）h。采用MALDI-TOF MS鉴定报阳后单个新鲜菌落，与VITEK 2 Compact全自动细菌鉴定方法的符合率达100%，从血培养报阳开始计算，平均鉴定时间为（22.0±0.6）h。结论 PNA-FISH方法鉴定白念珠菌，设备简单，鉴定结果可靠，相较于传统鉴定方法和MALDI-TOF MS方法，鉴定时间显著缩短，有较好的临床应用前景。 |
| 关键词: 白念珠菌 鉴定 肽核酸-荧光原位杂交技术 基质辅助激光解吸电离飞行时间质谱分析 |
| DOI：10.3969/j.issn.1674-3806.2022.10.08 |
| 分类号:R 446.5 |
| 基金项目:昆明市卫生科技人才培养计划项目［编号：2019-sw（省）-06］ |
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| A study on the clinical application of rapid identification method of Candida albicans |
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YANG Chun-li, REN Bao-jun, XU Yue-xuan, et al.
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Department of Laboratory Medicine, the First Hospital of Kunming, Yunnan 650011, China
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| Abstract: |
| ［Abstract］ Objective To explore the clinical application of peptide nucleic acid-fluorescence in situ hybridization(PNA-FISH) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry(MALDI-TOF MS) in rapid identification of Candida albicans. Methods One hundred strains of Candida albicans were identified by VITEK 2 Compact automatic bacteria identification instrument YST card, and simulated positive samples by blood culture were used. The positive samples were identified by PNA-FISH directly. A single fresh colony was transferred and cultured to complete MALDI-TOF MS identification. The results of identifying Candida albicans and the average identification time were compared between the two methods. The coincidence rate between the methods and VITEK 2 Compact automatic bacterial system in identifying Candida albicans were calculated. Results In the 100 simulated blood culture samples of Candida albicans, the coincidence rate between the identification result of PNA-FISH and that of VITEK 2 Compact automatic bacterial identification method was 98%, and the average identification time was (2.5±0.5)hours. MALDI-TOF MS was used to identify the single fresh colony, which was isolated from positive samples. The coincidence rate between the identification result of MALDI-TOF MS and that of VITEK 2 Compact automatic bacterial identification method was 100%. The average identification time was (22.0±0.6) hours from the time of positive blood culture. Conclusion The equipments required for the PNA-FISH method are simple but its identification results are reliable. Compared with that of the traditional identification methods and MALDI-TOF MS method, the identification time of the PNA-FISH method is significantly shortened and the PNA-FISH method has a better clinical application prospect. |
| Key words: Candida albicans Identification Peptide nucleic acid-fluorescence in situ hybridization(PNA-FISH) technology Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry(MALDI-TOF MS) analysis |
