引用本文:阳春丽,任宝军,徐跃轩,卢 赞,钱 净,杨文迪,马永鑫,赵晓丽.快速鉴定白念珠菌的临床应用研究[J].中国临床新医学,2022,15(10):933-937.
【打印本页】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 117次   下载 244 本文二维码信息
码上扫一扫!
分享到: 微信 更多
快速鉴定白念珠菌的临床应用研究
阳春丽,任宝军,徐跃轩,卢 赞,钱 净,杨文迪,马永鑫,赵晓丽
650011 云南,昆明市第一人民医院检验科
摘要:
[摘要] 目的 探究肽核酸-荧光原位杂交(PNA-FISH)、基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)快速鉴定白念珠菌的临床应用价值。方法 采用经VITEK 2 Compact全自动细菌鉴定仪YST卡鉴定白念珠菌100株,模拟血培养阳性标本。对报阳的标本直接进行PNA-FISH鉴定,再转种培养出单个新鲜菌落,完成MALDI-TOF MS鉴定。记录两种方法鉴定白念珠菌结果和鉴定所需时间,计算其与VITEK 2 Compact全自动细菌鉴定方法的符合率。结果 100株白念珠菌模拟血培养样本,PNA-FISH鉴定结果与VITEK 2 Compact全自动细菌鉴定方法的符合率为98%,平均鉴定时间为(2.5±0.5)h。采用MALDI-TOF MS鉴定报阳后单个新鲜菌落,与VITEK 2 Compact全自动细菌鉴定方法的符合率达100%,从血培养报阳开始计算,平均鉴定时间为(22.0±0.6)h。结论 PNA-FISH方法鉴定白念珠菌,设备简单,鉴定结果可靠,相较于传统鉴定方法和MALDI-TOF MS方法,鉴定时间显著缩短,有较好的临床应用前景。
关键词:  白念珠菌  鉴定  肽核酸-荧光原位杂交技术  基质辅助激光解吸电离飞行时间质谱分析
DOI:10.3969/j.issn.1674-3806.2022.10.08
分类号:R 446.5
基金项目:昆明市卫生科技人才培养计划项目[编号:2019-sw(省)-06]
A study on the clinical application of rapid identification method of Candida albicans
YANG Chun-li, REN Bao-jun, XU Yue-xuan, et al.
Department of Laboratory Medicine, the First Hospital of Kunming, Yunnan 650011, China
Abstract:
[Abstract] Objective To explore the clinical application of peptide nucleic acid-fluorescence in situ hybridization(PNA-FISH) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry(MALDI-TOF MS) in rapid identification of Candida albicans. Methods One hundred strains of Candida albicans were identified by VITEK 2 Compact automatic bacteria identification instrument YST card, and simulated positive samples by blood culture were used. The positive samples were identified by PNA-FISH directly. A single fresh colony was transferred and cultured to complete MALDI-TOF MS identification. The results of identifying Candida albicans and the average identification time were compared between the two methods. The coincidence rate between the methods and VITEK 2 Compact automatic bacterial system in identifying Candida albicans were calculated. Results In the 100 simulated blood culture samples of Candida albicans, the coincidence rate between the identification result of PNA-FISH and that of VITEK 2 Compact automatic bacterial identification method was 98%, and the average identification time was (2.5±0.5)hours. MALDI-TOF MS was used to identify the single fresh colony, which was isolated from positive samples. The coincidence rate between the identification result of MALDI-TOF MS and that of VITEK 2 Compact automatic bacterial identification method was 100%. The average identification time was (22.0±0.6) hours from the time of positive blood culture. Conclusion The equipments required for the PNA-FISH method are simple but its identification results are reliable. Compared with that of the traditional identification methods and MALDI-TOF MS method, the identification time of the PNA-FISH method is significantly shortened and the PNA-FISH method has a better clinical application prospect.
Key words:  Candida albicans  Identification  Peptide nucleic acid-fluorescence in situ hybridization(PNA-FISH) technology  Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry(MALDI-TOF MS) analysis