| 摘要: |
| ［摘要］ 目的 研究NIX蛋白的表达变化与熊去氧胆酸（UDCA）抑制人肝星状细胞（HSC）活化作用的关系。方法 人肝星状细胞-LX2细胞实验分为四组：溶媒对照组、UDCA（0.5 μM）组、TGF-β1（5 ng/ml）组、TGF-β1（5 ng/ml）+UDCA（0.5 μM）组。通过Western blot检测比较各组间α-SMA、NIX的表达差异。结果 TGF-β1刺激LX2细胞后，α-SMA、NIX表达分别较对照组增加60.9%、51.0%；UDCA抑制TGF-β1活化LX2细胞后，α-SMA的表达抑制率达55.6%，与其显著下调NIX的表达呈正相关。结论 UDCA下调HSC细胞NIX蛋白的表达，可能为UDCA抗肝纤维化的药理新机制。 |
| 关键词: 肝星状细胞 α平滑肌肌动蛋白 熊去氧胆酸 NIX |
| DOI：10.3969/j.issn.1674-3806.2018.02.01 |
| 分类号:R 975 |
| 基金项目:国家自然科学基金资助项目（编号：81360077）；广西消化疾病临床医学研究中心建设项目（编号：桂科AD17129027） |
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| An in vitro research of ursodesoxycholic acid inhibiting the activation of hepatic stellate cell and the expression of NIX protein |
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TENG Lin-hua, LIU Qi-lin, LIU Ze-feng, et al.
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Department of Gastroenterology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To study the relationship between the expression changes of NIP3-like protein X（NIX） in hepatic stellate cell(HSC) and the inhibitory role of ursodesoxycholic acid（UDCA） on activation of HSC.Methods HSC-LX2 cells were divided into four groups: vehicle control group,UDCA(0.5 μM) group, TGF-β1(5 ng/ml) group, and TGF-β1(5 ng/ml)+UDCA(0.5 μM) group. The expressions of α-SMA and NIX were detected by Western blot and were compared among the different groups.Results After HSC-LX2 cells were stimulated by TGF-β1, the expression of α-SMA in LX2 cells was 60.9% higher than that in the control group, and the expression of NIX was simultaneously elevated with an increase of 51.0%. The expression of a-SMA was inhibited 55.6% by UDCA in LX2 cells which were activated by TGF-β1, and that was positively correlated with the significantly down-regulated expression of NIX.Conclusion UDCA reduces the expression of NIX protein in HSC, which might be one of the pharmacological mechanisms of UDCA to counteract liver fibrosis. |
| Key words: Hepatic stellate cell(HSC) α-smooth muscle actin（α-SMA） Ursodesoxycholic acid（UDCA） NIP3-like protein X（NIX） |
