| 摘要: |
| ［摘要］ 目的 筛选影响卵巢癌发生发展的关键基因，并分析其作用机制。方法 利用高通量基因表达（GEO）数据库、癌症基因图谱（TCGA）数据库及KMplot数据库筛选卵巢癌关键基因，分析其与患者临床病理学特征的关系，同时使用GESA进行信号通路聚类分析，并使用TIMER数据库确定与之密切相关的基因，通过STRING数据库绘制互作网络图，预测其作用机制。结果 通过筛选共得到13个差异基因，其中ARX和FAM150B与患者预后有关，且其表达量越低，患者总生存期越短（P<0.01）。进一步分析发现ARX和FAM150B的表达量还与IL-6、PIK3R2、PIK3CD、VAV1、PIK3CA、RAC2、PIK3R1及MAPK1的表达量相关（P<0.05）。使用STRING数据库分析发现ARX和FAM150B通过TUBA1A、EGFR、PLXNA2对以上基因进行调控。结论 ARX和FAM150B在卵巢癌组织中呈低表达状态，它们的表达量和患者的预后密切相关。ARX和FAM150B可对一系列基因进行调控，从而减弱机体的抗肿瘤免疫反应，促进卵巢癌的发生发展。 |
| 关键词: 卵巢癌 高通量基因表达数据库 差异基因 ARX FAM150B |
| DOI：10.3969/j.issn.1674-3806.2021.02.13 |
| 分类号:R 737.31 |
| 基金项目: |
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| Screening key genes in occurrence and development of ovarian cancer by bioinformatics |
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YU Zhan-peng, PENG Liang, JIANG Qiao
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Department of Laboratory Medicine, the Fifth Affiliated Hospital of Guangzhou Medical University, Guangdong 510700, China
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| Abstract: |
| ［Abstract］ Objective To screen the key genes that affect the occurrence and development of ovarian cancer and to analyze their mechanisms of action. Methods The key genes of ovarian cancer were screened by using Gene Expression Omnibus(GEO), the Cancer Genome Atlas(TCGA) and Kaplan-Meier Plotter(KMplot) databases, and their relationship with the patients′ clinicopathological characteristics was analyzed. At the same time, GESA was used for cluster analysis of signal pathways, and the TIMER database was used to identify the genes closely related to it. The interaction network was mapped through the STRING database to predict its mechanism of action. Results A total of 13 differentially expressed genes were obtained through screening, among which Aristaless Related Homeobox(ARX) and Family with Sequence Similarity 150 Member B(FAM150B) were related to the prognosis of the patients, and the lower their expression levels, the shorter the overall survival time of the patients(P<0.01). Further analysis found that the expression levels of ARX and FAM150B were also related to the expression levels of interleukin-6(IL-6), Phosphoinositide-3-Kinase Regulatory Subunit 2(PIK3R2), PIK3CD, VAV1, PIK3CA, RAC2, PIK3R1, and MAPK1(P<0.05). The analysis of STRING database showed that ARX and FAM150B regulated the above genes through TUBA1A, EGFR and PLXNA2. Conclusion The expression levels of ARX and FAM150B is low in ovarian cancer tissues, and their expression levels are closely related to the prognosis of the patients. ARX and FAM150B can regulate a series of genes, thereby weakening the body′s anti-tumor immune response and thus promoting the occurrence and development of ovarian cancer. |
| Key words: Ovarian cancer Gene Expression Omnibus(GEO) Database Differentially expressed genes(DEGs) Aristaless Related Homeobox(ARX) Family with Sequence Similarity 150 Member B(FAM150B) |
