| 摘要: |
| ［摘要］ 目的 探讨尾型同源盒转录因子2(CDX2)、黏蛋白2(MUC2)在胃黏膜肠上皮化生组织中的表达情况及其临床意义。方法 选择2011年1月至2014年5月于宁夏医科大学总医院消化内科就诊并行内镜活检的152例患者，根据病理检查结果将其分为非萎缩性胃炎组（32例）、萎缩性胃炎组（31例）、低级别上皮内瘤变组（24例）、高级别上皮内瘤变组（5例）、胃癌组（17例）和胃黏膜肠上皮化生（IM）组（43例）。应用免疫组织化学法检测其胃黏膜组织中CDX2、MUC2的表达情况，用酶联免疫吸附法（ELISA）检测患者血清幽门螺杆菌(Hp）-IgG水平，分析CDX2、MUC2与Hp-IgG检测结果的一致性。结果 6组的CDX2、MUC2阳性率比较差异均有统计学意义（P<0.05）。其中IM组的CDX2、MUC2阳性率最高，显著高于其他5组（P<0.05）。IM组Hp-IgG阳性率高于胃炎组，差异有统计学意义（65.12% vs 42.50%； χ2=4.270，P=0.039）。Kappa一致性分析结果显示，在IM组中CDX2与Hp-IgG的表达情况具有一致性（P<0.05），但胃炎组CDX2与Hp-IgG的表达情况一致性不显著（P>0.05）。IM组和胃炎组MUC2与Hp-IgG的表达情况一致性不显著（P>0.05）。结论 CDX2、MUC2在IM组织中的阳性表达率高于其他性质的胃黏膜组织，Hp感染可能通过诱导CDX2的表达而引起IM的发生。 |
| 关键词: 胃黏膜肠上皮化生 尾型同源盒转录因子2 黏蛋白2 幽门螺杆菌 |
| DOI：10.3969/j.issn.1674-3806.2022.02.06 |
| 分类号:R 573.9 |
| 基金项目:宁夏回族自治区重点研发计划重大（重点）项目（编号：2018BEG02007） |
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| A study on the expressions of CDX2 and MUC2 in gastric intestinal metaplasia tissues and their clinical significances |
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YANG Jia-li, WANG Zhen, LIU Fei, et al.
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Department of Gastroenterology, General Hospital of Ningxia Medical University, Yinchuan 750000, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expressions of caudal-related homeobox transcription factor 2(CDX2) and mucin 2(MUC2) in gastric intestinal metaplasia tissues and their clinical significances. Methods A total of 152 patients who underwent endoscopic biopsy in Department of Gastroenterology, General Hospital of Ningxia Medical University from January 2011 to May 2014 were selected and divided into non-atrophic gastritis group(32 cases), atrophic gastritis group(31 cases), low-grade intraepithelial neoplasia group(24 cases), high-grade intraepithelial neoplasia group(5 cases), gastric cancer group(17 cases) and gastric intestinal metaplasia(IM) group(43 cases). The expressions of CDX2 and MUC2 in gastric mucosa tissues were detected by immunohistochemistry method, and the serum level of Helicobacter pylori(Hp)-IgG was detected by enzyme-linked immunosorbent assay(ELISA) method. The consistency of CDX2, MUC2 and Hp-IgG detection results was analyzed. Results There were significant differences in the positive rates of CDX2 and MUC2 among the six groups(P<0.05). Among the six groups, the positive rates of CDX2 and MUC2 in the IM group were the highest, and were significantly higher than those in the other five groups(P<0.05). The positive rate of Hp-IgG in the IM group was higher than that in the gastritis group, and the difference was statistically significant(65.12% vs 42.50%; χ2=4.270, P=0.039). The results of Kappa consistency analysis showed that the expressions of CDX2 and Hp-IgG were consistent in the IM group(P<0.05), but the expressions of CDX2 and Hp-IgG in the gastritis group were not significantly consistent(P>0.05). The expressions of MUC2 and Hp-IgG in the IM group and the gastritis group were not significantly consistent(P>0.05). Conclusion The positive expression rates of CDX2 and MUC2 in the IM tissues are higher than those in other gastric mucosal tissues. Hp infection may cause the occurrence of IM by inducing the expression of CDX2. |
| Key words: Gastric intestinal metaplasia(IM) Caudal-related homeobox transcription factor 2(CDX2) Mucin 2(MUC2) Helicobacter pylori(Hp) |
