| 摘要: |
| ［摘要］ 目的 分析溃疡性结肠炎(UC)患者血清及肠黏膜组织中miR-200b和miR-655的表达水平，探讨其与疾病活动度的关系。方法 选择2016年10月至2019年6月该院消化内科收治的UC患者36例，根据改良的Mayo评分系统将其分为内镜下活动期组（20例）和内镜下愈合期组（16例），另选择同期健康志愿者20名作为对照组。应用实时荧光定量聚合酶链反应（RT-qPCR）法检测肠黏膜组织及血清样本miR-200b、miR-655的表达水平，并进行三组间比较。结果 对照组肠黏膜miR-200b、miR-655表达水平显著高于内镜下活动期组和内镜下愈合期组（P<0.05），而内镜下活动期组与内镜下愈合期组比较差异无统计学意义（P>0.05）。内镜下活动期组血清中miR-200b、miR-655水平高于内镜下愈合期组和对照组，差异有统计学意义（P<0.05），但后两组血清miR-200b、miR-655水平比较差异无统计学意义（P>0.05）。结论 血清miR-200b、miR-655表达水平有可能间接反映UC患者的肠黏膜损伤情况。 |
| 关键词: miR-200b miR-655 溃疡性结肠炎 肠黏膜 内镜 疾病活动度 |
| DOI：10.3969/j.issn.1674-3806.2022.02.10 |
| 分类号:R 574.62 |
| 基金项目:广西自然科学基金项目（编号：2015GXNSFAA139222）；广西医疗卫生适宜技术开发与应用推广项目（编号：S2017073）；广西桂林市科学研究与技术开发项目（编号：20190218-8-2）；广西壮族自治区南溪山医院攻关项目（编号：NY20170002） |
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| A study on the expression levels of miR-200b and miR-655 in intestinal mucosal tissues and serum of patients with ulcerative colitis and their clinical significances |
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LUO Wen-jie, ZHANG Qi-fang, ZHANG Hai-lian, et al.
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Department of Gastroenterology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin 541002, China
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| Abstract: |
| ［Abstract］ Objective To analyze the expression levels of micro-ribonucleic acid-200b(miR-200b) and micro-ribonucleic acid-655(miR-655) in the serum and intestinal mucosal tissues of patients with ulcerative colitis(UC), and to explore their relationships with disease activity. Methods Thirty-six UC patients admitted to Department of Gastroenterology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region from October 2016 to June 2019 were selected. According to the improved Mayo scoring system, they were divided into the endoscopic active group(20 cases) and the endoscopic healing group(16 cases), and other 20 healthy volunteers during the same period were selected as the control group. Real-time quantitative polymerase chain reaction(RT-qPCR) method was used to detect the expression levels of miR-200b and miR-655 in intestinal mucosal tissues and serum samples, and these expression levels were compared among the three groups. Results The expression levels of miR-200b and miR-655 in the intestinal mucosa of the control group were significantly higher than those of the endoscopic active group and the endoscopic healing group(P<0.05), while there was no statistically significant difference between the endoscopic active group and the endoscopic healing group(P>0.05). The serum levels of miR-200b and miR-655 in the endoscopic active group were higher than those in the endoscopic healing group and the control group, and the differences were statistically significant(P<0.05). However, there were no significant differences in the serum levels of miR-200b and miR-655 between the latter two groups(P>0.05). Conclusion The expression levels of serum miR-200b and miR-655 may indirectly reflect the intestinal mucosal injury in UC patients. |
| Key words: Micro-ribonucleic acid-200b(miR-200b) Micro-ribonucleic acid-655(miR-655) Ulcerative colitis(UC) Intestinal mucosa Endoscopy Disease activity |
