| 摘要: |
| 【摘要】 目的：探讨抗凝剂依赖性假性血小板减少症患者血小板计数的有效纠正措施，达到准确计数此类患者外周血中血小板数量，为临床检验工作提供依据。 方法：选取抗凝剂依赖性假性血小板减少症患者1例，分别采集1份干燥红管无抗凝血3mL、1份肝素锂抗凝血3mL、1份枸橼酸钠抗凝血2mL和1份EDTA-K2抗凝血2mL外周血样本。无抗凝血采集后迅速上机检测；肝素锂、枸橼酸钠和EDTA-K2抗凝血采集后分别于0、15、30、60和120min上机检测。同时进行外周血涂片观察和血小板手工计数。 结果：多种抗凝剂都可以引起血小板聚集，导致血小板假性减少；0min无抗凝血检测的血小板结果与手工计数结果相比无明显差异，且镜检未见血小板聚集；0min肝素锂抗凝血检测的血小板结果与手工计数结果相比无明显差异，但镜检显示血小板有散在聚集，且15min之后聚集明显；枸橼酸钠和EDTA-K2抗凝血不同时间上机检测血小板结果与手工计数结果相比都存在明显差异，且标本采集后2小时内随时间延长血小板聚集程度加大，而EDTA-K2抗凝血聚集更明显；0min无抗凝血血常规检测结果与EDTA-K2抗凝血血常规检测结果除血小板外，其他血细胞计数无明显差异。 结论：对抗凝剂依赖性假性血小板减少症患者采用采集无抗凝剂标本且迅速上机检测的方法，不仅能避免外来物质干扰而且能有效纠正抗凝剂依赖性假性血小板减少，同时不影响其他血细胞计数，结果准确，既省时又节约人力物力且操作简单实用。 |
| 关键词: 抗凝剂 假性血小板减少 有效纠正 计数 |
| DOI： |
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| Correction of Platelet Count in a Patient with Anticoagulant Dependent Pseudothrombocytopenia |
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wangdong
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Wuhan Central South University Hospital
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| Abstract: |
| [Abstract] Objective: To explore effective corrective measures for platelet count in patients with anticoagulant-dependent pseudothrombocytopenia, so as to accurately count the number of platelet counts in the blood of patients with anticoagulant-dependent pseudothrombocytopenia and provide basis for clinical examination. Methods: One case of anticoagulant-dependent pseudothrombocytopenia was selected. One dry red tube with no anticoagulant 3mL, one Heparin lithium salt anticoagulant 3mL, one sodium citrate anticoagulant 2mL and one EDTA-K2 anticoagulant 2mL outer week blood samples were collected respectively. No anticoagulant was collected and tested on the computer quickly. Heparin lithium salt, sodium citrate and EDTA-K2 were collected and tested on the computer at 0, 15, 30, 60 and 120min respectively. At the same time, external week blood smear observation and platelet manual counting were performed. Results: Various anticoagulants can cause platelet aggregation, leading to pseudoreduction of platelets. There was no significant difference between the results of platelet count without anticoagulant test at 0min and manual count, and no platelet aggregation was found under microscopic examination. There was no significant difference between the results of platelet count by Heparin lithium salt anticoagulant test at 0min and manual count, but microscopic examination showed that platelets were scattered and aggregated, and the aggregation was obvious after 15min. Compared with manual counting results, the results of platelet detection by computer at different times of sodium citrate and EDTA-K2 anticoagulation are obviously different, and the degree of platelet aggregation increases with the extension of time within 2 hours after specimen collection, while EDTA-K2 anticoagulation aggregation is more obvious. There was no significant difference between the results of non-anticoagulant blood routine test at 0min and EDTA-K2 anticoagulant blood routine test except platelets. Conclusion: The anticoagulant-dependent pseudothrombocytopenia patients can not only avoid the interference of foreign substances but also effectively correct the anticoagulant-dependent pseudothrombocytopenia without affecting other blood cell counts by adopting the method of collecting anticoagulant-free specimens and testing on the computer quickly, with accurate results, saving time, manpower and material resources, and simple and practical operation. |
| Key words: Anticoagulant Pseudothrombocytopenia Effective correction count |
