| 摘要: |
| 目的 探究体内钙粘蛋白13(cadherin-13,CDH13)表达下调对膀胱肿瘤的生长及磷脂酰肌醇3激酶( phosphatidylinositol 3 kinase,PI3K)/蛋白激酶B(protein kinase B,Akt )蛋白表达的影响。方法 应用CDH13稳定低表达的膀胱癌5637细胞建立裸鼠成瘤模型,肿瘤出现后定时测量正常对照组(1× PBS)、阴性对照组(control shRNA Plasmid)和实验组(CDH13 shRNA Plasmid)三组的肿瘤体积并绘制生长曲线,并采用免疫印迹技术检测肿瘤组织中CDH13、PI3K及p-Akt等蛋白表达的改变。结果 与正常对照组和阴性对照组相比,在膀胱癌5637细胞建立的裸鼠成瘤模型中CDH13表达下调后,肿瘤体积增大(P ＜ 0.05),PI3K和p-Akt的蛋白表达明显增加(P ＜ 0.05),差异均有统计学意义。结论 在膀胱癌5637细胞建立的裸鼠成瘤模型中,CDH13表达下调可能通过PI3K/Akt通路促进肿瘤生长。 |
| 关键词: 膀胱癌 肿瘤生长 CDH13 PI3K/Akt |
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| 基金项目:徐州市医学青年后备人才项目(编号：2014007)；江苏大学临床科技发展(编号：JLY20160064)；江苏省青年医学重点人才项目(编号：QNRC2016395)；江苏省卫生计生委科研课题(编号：Z2017015)；江苏省第五期“333工程”科研资助立项项目(编号：BRA2017296)；徐州市科技计划项目(编号：KC17200)。 |
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| The study of down-regulation of cadherin-13 (CDH13) expression on the growth of tumor and the expression of PI3K/Akt in vivo |
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Xuzhou Cancer Hospital
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| Abstract: |
| Objective To explore the effect of down-regulation of cadherin-13 (CDH13) expression on the growth of tumor and the expression of PI3K/Akt in vivo. Methods The nude mouse model of bladder cancer was established by screening the bladder cancer 5637 cells with the down-regulation of CDH13 expression. The nude mice (n=21)were divided into normal control group (1×PBS), negative control group (control shRNA Plasmid) and experimental group (CDH13 shRNA Plasmid), 7 mice in each group. The tumor volume and the growth curve were measured on schedule. The protein levels of CDH13, PI3K and p-Akt was detected by immunoblotting. Results After the protein level of CDH13 was down-regulated in the nude mouse model of bladder cancer 5637 cells, the tumor volume increased (P ＜ 0.05) and the protein levels of PI3K and p-Akt were enhanced notably (P ＜ 0.05). Conclusion In the nude mouse model of bladder cancer 5637 cells, down-regulation of CDH13 may promotes tumor growth through the PI3K/Akt pathway. |
| Key words: bladder cancer the growth of tumor CDH13 PI3K/Akt |
