引用本文:刘子歌,张晨,宋国瑞,李昃鹏,郭凤英,李燕,刘心蕊,杨超.IL-34、RANKL与OPG在无菌性松动患者界膜组织中的表达[J].中国临床新医学,0,():-.
.IL-34、RANKL与OPG在无菌性松动患者界膜组织中的表达[J].中国临床新医学,0,():-.
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IL-34、RANKL与OPG在无菌性松动患者界膜组织中的表达
刘子歌1, 张晨1, 宋国瑞1, 李昃鹏2, 郭凤英3, 李燕3, 刘心蕊4, 杨超5
1.宁夏医科大学临床医学院;2.南华大学附属第一医院骨科;3.宁夏医科大学基础医学院;4.宁夏医科大学;5.银川国龙医院
摘要:
目的 通过研究白细胞介素34(IL-34)、核因子κB受体活化因子配体(RANKL)、骨保护素(OPG)在无菌性松动患者血清和界膜组织中的表达,并探讨其与无菌性松动的关系。方法 收集在2018年10月至2020年6月与宁夏医科大学总医院行人工髋关节置换术的患者,共20例,分别纳入无菌性松动组和对照组各10例。实时定量聚合酶链式反应(RT-PCR)、酶联免疫吸附试验(ELISA)检测界膜组织中IL-34、RANKL与OPG的表达水平。结果 RT-PCR与ELISA的结果均显示无菌性松动组组中OPG、RANKL及IL?34水平及RANKL￥OPG值显著高于无菌性松动组,且差异有统计学意义(P <0.05)。结论 我们的结果显示IL-34参与了颗粒诱导的人工关节无菌性松动的病理过程,提示IL-34可能是抑制破骨细胞形成的潜在治疗靶标。IL-34与RANKL表达水平在无菌性松动患者中明显升高,OPG则在无菌性松动患者中表现出明显降低,IL-34、RANKL与OPG共同参与无菌性松动的发病过程。
关键词:  无菌性松动  人工关节置换术  IL-34  RANKL  OPG
DOI：
分类号:R318.17
基金项目:国家自然科学基金(81560364；81760405；81760395)； 宁夏自然科学基金重点项目(2018AAC02013)；宁夏医科大学校级课题重点项目(XZ2018014)
Expression of IL-34, RANKL and OPG in the bound membrane tissue of patients with aseptic loosening LIU Zi-ge, ZHANG Chen, SONG Guo-rui, et al. School of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, P．R．China
NingXia Medical University
Abstract:
Objective To investigate the expression of interleukin 34 (IL-34), nuclear factor κB receptor activating factor ligand (RANKL), and osteoprotegerin (OPG) in the serum and boundary membrane tissue of patients with aseptic loosening, and Explore its relationship with aseptic looseness. Methods A total of 20 patients who underwent artificial hip replacement surgery between October 2018 and June 2020 at the General Hospital of Ningxia Medical University were collected, and they were included in the aseptic loosening group and the control group, respectively. Real-time quantitative polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression levels of IL-34, RANKL and OPG in the membrane tissue. Results Both RT-PCR and ELISA results showed that the ratio of OPG, RANKL and IL?34 and RANKL\OPG in the aseptic loosening group was significantly higher than that of the aseptic loosening group, and the difference was statistically significant (P<0.05). Conclusion Our results indicate that IL-34 is involved in the pathological process of particle-induced aseptic loosening and may be a potential therapeutic target for inhibiting osteoclast formation. IL-34 and RANKL were significantly increased in patients with aseptic loosening, OPG was significantly reduced in patients with aseptic loosening, IL-34, RANKL and OPG jointly participated in the pathogenesis of aseptic loosening.
Key words:  Aseptic loosening  Artificial joint replacement  IL-34  RANKL  OPG