| 引用本文: | 刘子歌,宋国瑞,张晨,王强,郭高鹏.红霉素对聚甲基丙烯酸甲酯磨损颗粒刺激下MC3T3-E1细胞成骨作用的相关研究[J].中国临床新医学,0,():-. |
| 刘子歌.红霉素对聚甲基丙烯酸甲酯磨损颗粒刺激下MC3T3-E1细胞成骨作用的相关研究[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 本研究旨在探讨红霉素对聚甲基丙烯酸甲酯(PMMA)颗粒刺激MC3T3-E1胚胎成骨细胞成骨作用的影响,及其潜在的机制。方法 MC3T3-E1细胞用不同浓度红霉素预处理,然后用PMMA刺激。细胞活力通过乳酸脱氢酶(LDH)释放实验测定进行评估。细胞分化程度通过碱性磷酸酶(ALP)进行评估。通过RT-PCR检测Runx2、Osterix、OCN和NF-κB p65的基因表达。结果 PMMA颗粒刺激能够显著降低MC3T3-E1的细胞活力及成骨功能,而红霉素能够有效缓解PMMA颗粒对MC3T3-E1细胞的增殖、生存能力及分化的影响； PMMA颗粒刺激可以降低MC3T3-E1细胞活力,通过改变Runx2、Osterix和OCN成骨细胞分化相关蛋白及NF-κB的表达影响MC3T3-E1细胞的成骨分化作用；红霉素能够有效缓解PMMA颗粒对MC3T3-E1细胞的增殖、生存能力及分化的影响。结论 红霉素可有效缓解PMMA磨损颗粒对MC3T3-E1胚胎成骨细胞的活力和成骨分化的刺激作用,且部分是通过下调NF-κB信号转导通路途径。 红霉素对PMMA磨损颗粒刺激下MC3T3-E1成骨分化抑制起保护作用。 |
| 关键词: 红霉素 聚甲基丙烯酸甲酯 无菌性松动 磨损颗粒 成骨细胞 |
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| 基金项目:国家自然科学基金资助项目(81760405,81760395,82060408);宁夏自然科学基金重点项目(2018AAC02013); |
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| Research on the effect of erythromycin on the osteogenesis of MC3T3-E1 cells stimulated by polymethylmethacrylate wear particles. Liu Zige. Department of Orthopaedics, General Hospital of Ningxia Medical University, NingXia 750004 China |
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刘子歌
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NingXia Medical University
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| Abstract: |
| Objective This study aimed to investigate the effect of erythromycin on the osteogenesis of MC3T3-E1 embryonic osteoblasts stimulated by polymethylmethacrylate (PMMA) particles and its underlying mechanism. Methods MC3T3-E1 cells were pretreated with different concentrations of erythromycin, and then stimulated with PMMA (1mg/mL). Cell viability was evaluated by the lactate dehydrogenase (LDH) release test. The degree of cell differentiation is assessed by alkaline phosphatase (ALP). The gene expression of Runx2, Osterix, OCN and NF-κB p65 was measured by RT-PCR. Results PMMA particles stimulation can significantly reduce the cell viability and osteogenic function of MC3T3-E1, while erythromycin can effectively alleviate the effects of PMMA particles on the proliferation, viability and differentiation of MC3T3-E1 cells; cell viability, by changing Runx2, The expression of Osterix and OCN osteoblast differentiation-related proteins and NF-κB affect the osteogenic differentiation of MC3T3-E1 cells; erythromycin can effectively alleviate the effects of PMMA particles on the reproduction, viability and differentiation of MC3T3-E1 cells. Conclusion Erythromycin reduces the viability and osteogenic differentiation of PMMA wear particles on MC3T3-E1 embryonic osteoblasts, partly by down-regulating the NF-κB signal transduction pathway. Erythromycin can significantly improve the above effects, and has a protective effect on the inhibition of MC3T3-E1 osteogenic differentiation under the stimulation of PMMA wear particles. |
| Key words: Erythromycin Polymethylmethacrylate Aseptic loosening Wear particles Osteoblasts |
