| 摘要: |
| ［摘要］ 目的 探讨应急性卵母细胞玻璃化冷冻技术在辅助生殖技术中对胚胎质量及发育潜力的影响。方法 回顾性分析2010年4月至2023年5月于广西壮族自治区生殖医院行应急性卵母细胞玻璃化冷冻的55例患者（55个周期，冻卵组）的临床资料，另随机抽选同期以新鲜卵母细胞行卵胞浆内单精子注射（ICSI）的201例患者（201个周期）作为对照组。分析冷冻卵母细胞复苏后行ICSI的结果情况，并比较两组受精情况、胚胎发育情况及临床妊娠结局。结果 55个周期进行卵母细胞冷冻，其中12个周期未解冻仍处于冷冻状态，其余43个周期共450枚卵母细胞全部解冻，存活400枚，复苏存活率为88.89%。冻卵组的移植胚胎数多于对照组，但其优质胚胎率、囊胚形成率和可利用囊胚率低于对照组，差异有统计学意义（P<0.05）。两组受精率、正常卵裂率比较差异无统计学意义（P>0.05）。冻卵组无可移植胚胎周期率、临床妊娠率、异位妊娠率低于对照组，但差异无统计学意义（P>0.05）。冻卵组种植率显著低于对照组（P<0.05）。两组流产率、活产率、分娩孕周、新生儿出生体重及出生缺陷率比较差异无统计学意义（P>0.05）。结论 应急性卵母细胞玻璃化冷冻可能对胚胎有一定损伤，但是仍可获得与新鲜卵母细胞相近的临床结局，是取卵日无可用精子补救措施和保存女性生育力的有效方法。 |
| 关键词: 卵母细胞 玻璃化冷冻 卵胞浆内单精子注射 临床结局 |
| DOI：10.3969/j.issn.1674-3806.2025.04.16 |
| 分类号:R 711.6 |
| 基金项目:广西卫生健康委员会科研课题（编号：Z-A20220373） |
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| Effects of emergency oocyte vitrification on embryo quality and developmental potential in assisted reproductive technology |
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GONG Fangqiang, HUANG Hua, LAO Yanping, SHI Li, NIU Xiangli
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The Reproduction Hospital of Guangxi Zhuang Autonomous Region, Nanning 530001, China
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| Abstract: |
| ［Abstract］ Objective To explore the effects of emergency oocyte vitrification technique on embryo quality and developmental potential in assisted reproductive technology. Methods The clinical data of 55 patients(55 cycles, egg freezing group) who underwent emergency oocyte vitrification in the Reproduction Hospital of Guangxi Zhuang Autonomous Region from April 2010 to May 2023 were retrospectively analyzed, and other 201 patients(201 cycles) who underwent intracytoplasmic sperm injection(ICSI) using fresh oocytes during the same period were randomly selected as control group. The results of ICSI after resuscitation of frozen oocytes were analyzed, and fertilization, embryo development and clinical pregnancy outcomes were compared between the two groups. Results A total of 55 cycles of oocytes were frozen, of which 12 cycles were still frozen without being thawed, and the remaining 43 cycles had a total of 450 oocytes and all of them were thawed, of which 400 oocytes survived, and the recovery rate was 88.89%. The number of transplanted embryos in the egg freezing group was larger than that in the control group, but the high-quality embryo rate, blastocyst formation rate and available blastocyst rate were lower than those in the control group, with significant differences between the two groups(P<0.05). There were no significant differences in fertilization rate and normal cleavage rate between the two groups(P>0.05). The cycle rate of untransplantable embryos, clinical pregnancy rate and ectopic pregnancy rate in the egg freezing group were lower than those in the control group, but the differences were not statistically significant(P>0.05). The transplanting rate of the egg freezing group was significantly lower than that of the control group(P<0.05). There were no significant differences in abortion rate, live birth rate, gestational week, birth weight and birth defect rate between the two groups(P>0.05). Conclusion Emergency oocyte vitrification may cause some damages to the embryos, but their clinical outcomes are still similar to those of fresh oocytes. Emergency oocyte vitrification is a remedy for the absence of sperms on the day of egg retrieval and an effective way to preserve female fertility. |
| Key words: Oocyte Vitrification Intracytoplasmic sperm injection(ICSI) Clinical outcome |
