| 摘要: |
| ［摘要］ 目的 探讨女性尖锐湿疣（CA）患者感染人类乳头瘤病毒（HPV）的基因类型和分布。方法 采用荧光定量聚合酶链反应（FQ-PCR）方法检测256例CA患者疣体组织或分泌物中的HPV类型。结果 HPV6、11型阳性率为93%（238/256）, HPV16、18型阳性率为15.2%（39/256）, HPV6、11型和HPV16、18同时阳性为10.9%（28/256）。定量检测病毒载量最高为1.0×108 copies/ml，最低为2.2×104 copies/ml。结论 本地区尖锐湿疣患者感染以HPV6、11低危型为主，少数为HPV16、18高危型；荧光PCR检测技术具有灵敏度高、特异性强及检测时间短等特点，能够及时准确的为HPV患者临床治疗及预后判断提供可靠的依据。 |
| 关键词: FQ-PCR 女性尖锐湿疣 HPV6、11型 HPV16、18型 |
| DOI：10.3969/j.issn.1674-3806.2009.09.14 |
| 分类号:R 446.69 |
| 基金项目: |
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| HPV genotyping for women patients with CA by FQ-PCR method |
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QIN Gui-fang, LIU Xin, ZHAO Lin
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Department of laboratroy, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the types and distribution of HPV genes in female condyloma (CA) patients infected with human papilloma virus(HPV).Methods Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detecte the typos of HPV genes in wart tissue or secretion from 256 CA patients.Results HPV6, 11 type-positive rate was 93% (238/256), HPV16, 18 type-positive rate was 15.2% (39/256), both positive rate of HPV6, 11 and HPV16, 18 was 10.9% (28/256). Quantitative detection of viral load showed, the maximum viral load was1.0 × 108 copies/ml, with a minimum of 2.2 × 104 copies/ml.Conclusion The patients with condyloma in this region were mainly infected by HPV6, 11 low-risk type, with a small number fo HPV16, 18 high-risk type. Fluorescence quantitative PCR detection technique, with high sensitivity, specificity and detection characteristics of a short time, can timely and accurately provide a reliable basis for the clinical treatment and the prognosis of patients with HPV. |
| Key words: FQ-PCR Female CA patients, HPV6,11 HPV16,18 |
