| 摘要: |
| ［摘要］ 目的 对微柱凝胶法和固相抗体法血小板抗体的检测方法进行比较，以选择更佳的方法进行检测。方法 采用微柱凝胶法和固相抗体（Capture-P）法分别测定154例多次输注血小板效果不佳的病人的血小板抗体，用pems统计软件进行kappa一致性检验。结果 微柱凝胶方法检出强阳性15例，阳性63例，阴性80例；Capture-P法检出强阳性5例，阳性45例，阴性104例，kappa指数0.63566。结论 两种检测方法一致性良好, 但微柱凝胶法试剂价格低廉、检测时间短，适宜基层单位推广。 |
| 关键词: 微柱凝胶 固相抗体 血小板抗体 |
| DOI：10.3969/j.issn.1674-3806.2009.09.24 |
| 分类号:R 446.11 |
| 基金项目: |
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| The consistency checking for two methods of platelet antibody detection |
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ZHANG Zhi-zhe
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Nanning the First People′s Hespital, Nanning Guangxi 530022, China
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| Abstract: |
| ［Abstract］ Objective To compare the methods for platelet antibody detection and have a better choice of detection mothods.Methods In 154 cases who had multiple platelet transfusion and didn′t have better effectiveness, the platelet antibody were detected by the methods of microcolumn gelatum and the method of insolubilized antibody Capture-P and the pems sofeware of statistics methods was used for kappa consistency checking.Results Fifteen cases were strong positive, 63 cases were positive and 80 cases were nagative by the methods of microcolumn gelatum. On the other hand, 5 cases were strong positive, 45 cases were positive and 104 cases were nagative by the method of insolubilized antibody Capture-P. kappa index number was 0.63566.Conclusion There is a better concordance of two methods, and agent of the methods of microcolumn gelatum has cheapper price and shorter checking time, it is suitable for primary level hospitals. |
| Key words: Microcolumn gelatum Insolubilized antibody Platelet antibody |
