| 摘要: |
| ［摘要］ 目的 探讨一种高效、经济、稳定的肝星状细胞的分离方法，为肝纤维化研究提供细胞模型。方法 应用链霉蛋白酶、胶原酶灌流，以Nycodenz为分离介质，采用密度梯度离心法分离大鼠的肝星状细胞。结果 肝星状细胞的获得率为（3.2±0.67）×107/只、存活率为90%以上。结论 该法是一种较理想的分离肝星状细胞的方法。 |
| 关键词: 肝星状细胞 细胞分离 肝纤维化 |
| DOI：10.3969/j.issn.1674-3806.2010.12.01 |
| 分类号:R 575 |
| 基金项目:国家自然科学基金资助项目（编号：30960145）;人社部资助留学人员科技活动择优项目（编号：200903）;广西自然科学基金资助项目（编号：0135035）;广西卫生厅重点科研课题（编号：桂卫重200958） |
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| A reliable method for the isolation and culture of rat hepatic stellate cells |
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ZHANG Guo, HUANG Jun-juan,ZHANG Fa-can, et al.
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Department of Gastroenterology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To develop a productive,inexpensive and stable method for the isolation and culture of rat hepatic stellate cells (HSCs) as a cell model for the studies of hepatic fibrosis.Methods Rat HSCs were isolated by liver perfusion with pronase and collagenase followed by density gradient centrifugation with Nycodenz. Results The harvest rate of hepatic stellate cells was （3.2±0.67）×107 cells per liver and viability rate was more than 90%.Conclusion This method is an ideal option for the isolation and culture of rat HSCs. |
| Key words: Hepatic stellate cells (HSCs) Cell isolation Hepatic fibrosis |
