| 摘要: |
| ［摘要］ 目的 了解T-bet基因在头颈部鳞癌患者颈淋巴结中的表达特征和意义。方法 取喉癌、舌癌和下咽癌患者颈清扫新鲜和石蜡包埋的淋巴结标本分别用逆转录聚合酶链反应和免疫组化检测T-bet RNA和蛋白水平的表达。结果 在鳞癌患者阳性转移淋巴结T-bet mRNA表达强度为（0.118±0.101）；在阴性淋巴结中为（0.307±0.121）,两者比较，P<0.05。喉癌、舌癌和下咽癌患者阳性颈淋巴结中T-bet蛋白阳性表达率分别为42.11%、25.00%和10.00%;阴性淋巴结组织中分别为65.79%、62.50%和50.00%,P均<0.05。在颈部阳性淋巴结中的T-bet表达阳性率与肿瘤部位、分化程度无关，Ⅲ期鳞癌患者的颈部阳性淋巴结中的T-bet表达阳性率明显高于Ⅳ期患者。三种鳞癌患者中，T-bet表达阳性者5年生存状况比阴性者好,[FK(W]P均<[FK)]0.05。结论 T-bet表达阳性的淋巴结患者的生存期延长；T-bet表达阳性的淋巴结受肿瘤转移侵犯的机会减少；T-bet阳性表达率可反映肿瘤的分子分期。 |
| 关键词: 鳞癌 转录因子 转移 |
| DOI：10.3969/j.issn.1674-3806.2011.03.13 |
| 分类号:R 739.91 |
| 基金项目: |
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| The express pattern of T-bet gene and its value in cervical lymph node of patients with squamous cell carcinoma of the head and neck |
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BAI Jian-wen, QU Shen-hong,LUO Chan-qiao,et al.
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Department of Surgery, Changde Tumor Hospital, Hunan 415125, China
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| Abstract: |
| ［Abstract］ Objective To investigate the express pattern of T-bet gene and its significance in cervical lymph node of patients with squamous cell carcinoma of the head and neck（SCCHN）.Methods The fresh and paraffin-imbedding cervical lymph node (CLN) samples of patients with squamous cell carcinoma of larynx, tongue and hypopharynx after radical neck dissection (RND) was respectively subjected to analysis of T-bet mRNA expression by semiquaitative reverse transcriptase-polymerase chain reaction (RT-PCR) and T-bet protein expression by immunohistochemistry.Results T-bet mRNA average express intensity in above CLN of SCCHN with positive and negative metastasis was respectively (0.118±0.101) and (0.307±0.121), between which the difference was statistically significant（P<0.05）. The positive express rate of T-bet protein in of CLN with positive metastasis in patients with carcinoma of larynx, tongue and hypopharynx was respectively 42.11%, 25.00% and 10.00%, and it was respectively 65.79%, 62.50% and 50.00% in patients with negative metastasis (all P<0.05). In all SCCHN patients, the five years survival rate of patients with positive express of T-bet was higher than that with negative express of T-bet (all P<0.05).Conclusion SCCHN patients with the positive express of T-bet in CLN has higher survival rate. The CLN with positive express of T-bet has less possibility to suffer from cancer metastasis. The positive express of T-bet can indicate the molecular stage of carcinoma. |
| Key words: Squamous cell carcinoma Transcription factors Metastasis |
