| 摘要: |
| ［摘要］ 目的 探讨4-乙酰氧基苯并恶唑-2-酮（AcO-BOA）对体外培养大鼠肝星状细胞(HSC-T6)增殖的影响。方法 将HSC-T6细胞分别在实验组(AcO-BOA浓度分别为0.008、0.04、0.2、1.0、5.0 mg/ml)及对照组(单纯培养液)中体外培养32 h后，应用四甲基偶氮唑盐法(MTT法)检测细胞增殖情况；荧光倒置显微镜下观察两组HSC-T6细胞的形态学变化。结果 实验组HSC-T6细胞的增殖率均低于对照组，浓度为0.008 mg/ml的实验组与对照组相比差异无统计学意义(P>0.05)；其他浓度的实验组分别与对照组比较差异有统计学意义(均P<0.05)。AcO-BOA对HSC-T6细胞的增殖抑制率均随着药物作用浓度的升高而逐渐升高；经吖啶橙染色后，在荧光倒置显微镜下观察，均可见细胞数量减少、体积缩小、核破裂、核浓缩等变化，且随着作用浓度的增加变化更加明显。结论 AcO-BOA对HSC-T6细胞具有抑制增殖的功效。 |
| 关键词: 4-乙酰氧基苯并恶唑-2-酮 肝星状细胞 吖啶橙 |
| DOI：10.3969/j.issn.1674-3806.2011.06.05 |
| 分类号:R 575.2 |
| 基金项目:广西自然科学基金资助项目（编号：0991123） |
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| Inhibitory effect of 4-acetoxy-2-benzoxazolone on rat hepatic stellate cell proliferation |
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FAN Hui, QI Ping, LIU Lin, et al.
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Pharmaceutical Department of Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To evaluate the effects of 4-acetoxy-2-benzoxazolone（AcO-BOA）on proliferation of rat hepatic stellate cells (HSC-T6) in vitro. Methods Rat HSC-T6 was incubated with different concentrations of AcO-BOA (0.008 mg/ml，0.04 mg/ml，0.2 mg/ml，1.0 mg/ml，5.0 mg/ml respectively) or without AcO-BOA for 32 hours, respectively. The proliferation rates of the HSC-T6 was determined by MTT assay. The morphological changes of the HSC-T6 were observed with inverted fluorenscence microscopy.Results MTT assay indicated that the proliferation rates of HSC-T6 induced with different concentrations of AcO-BOA were significantly reduced compared with the control group incubated in vitro for 32 h ( all P<0.05), except the group whose concentration was 0.008 mg/ml (P>0.05). AcO-BOA significantly inhibited the proliferation of HSC-T6 in a dose-dependent manner. After acridine orange staining, inverted fluorenscence microscopy showed the morphological changes of HSC-T6, including depletion in numbers, cells shrinkage, karyorrhexis and karyopycnosis, and the changes were more significant as the concentration increased.Conclusion The inhibitory effect on cell proliferation of AcO-BOA really exist. |
| Key words: 4-acetoxy-2-benzoxazolone Hepatic stellate cell Acridine orange |
