| 摘要: |
| ［摘要］ 目的 探讨柠檬酸钠（sodium citrate，CT）与三氧化二砷（As2O3，AS）联合应用对胃癌SGC-7901细胞凋亡及作用机制。方法 用sodium citrate（终浓度为5 mmol/L，CT5）和As2O3（终浓度依次为5 μmol/L，AS5）处理体外传代培养的胃癌SGC-7901细胞株。应用DAPI细胞核染色和流式细胞仪检测细胞凋亡和细胞周期的变化；RT-PCR法观察抗凋亡相关基因bcl-2表达的变化。结果 sodium citrate与As2O3联合应用相对于单用sodium citrate或As2O3可显著提高诱导胃癌细胞凋亡率（P<0.05）；与空白组相比，用药后G0/G1期细胞比例下降，G2/M期细胞比例上升,细胞周期阻滞于G2/M期，抗凋亡基因bcl-2表达明显下降。结论 sodium citrate与As2O3联合应用具有协同抗胃癌细胞的作用,其机制可能与细胞周期阻滞、增强诱导胃癌细胞凋亡以及调控凋亡相关基因的表达有关。 |
| 关键词: 胃癌 柠檬酸钠 三氧化二砷 凋亡 bcl-2基因 |
| DOI：10.3969/j.issn.1674-3806.2012.03.01 |
| 分类号:R 573 |
| 基金项目:广西自然科学基金资助项目（编号：0832118） |
|
| Anti-tumor effect of sodium citrate in combination with arsenic trioxide on gastric cancer cell line SGC-7901 in vitro |
|
ZHENG Bu-ping, ZHANG Xiao-dong, LU Yun-fei
|
|
Department of Gastrointestinal and Glands Surgery, the First Affiliated Hospital of Guangxi Medical University,Nanning 530021, China
|
| Abstract: |
| ［Abstract］ Objective To study the anti-tumor effects of sodium citrate combined with arsenic trioxide on human gastric cancer cell line SGC-7901 in vitro and to explore its possible mechanism.Methods The passaged and cultured gastric cancer cell line SGC-7901 in vitro were treated with sodium citrate ( final concentration was 5mmol/L，CT5) and(or)As2O3 (final concentration were 5 μmol/L，AS5).Cell proliferation was observed by fluorescent inversive microscopy; Cell apoptosis was explored by DAPI fluorescent stain, then the cell apoptosis rate and phases of cell cycle were determined by flow cytometry. The expression of apoptosis-related genes of bcl-2 was observed by RT-PCR.Results Sodium citrate combined with As2O3 had a stronger inhibition effect on human gastric cancer cell line SGC-7901 than sodium citrate or As2O3(P<0.05). Compared with blank group,the cell cycle distribution was altered and the ration of G0/G1 phase cell decreased and G2/M phase increased (P<0.05). After intervened by sodium citrate combined with As2O3,the expression of bcl-2 decreased（P<0.05）.Conclusion Sodium citrate combined with As2O3 has the synergistic effects on gastric cancer cell line SGC-7901,and the mechanisms may be related to cell cycle blocking and apoptosis-inducing effects through regulating the expression of the related genes. |
| Key words: Gastric cancer Sodium citrate Arsenic trioxide Apoptosis bcl-2 |
