| 摘要: |
| ［摘要］ 目的 探讨广西麻鸭作为乙型肝炎病毒感染动物模型的可能性。方法 应用广西1 d龄雏麻鸭经腹腔感染鸭乙型肝炎病毒（DHBV），13 d后采用实时荧光定量PCR法检测麻鸭血清DHBV含量，筛选出DHBV强阳性鸭。结果 共检测148份麻鸭血清标本，其中DHBV阳性标本136份，阳性率为91.9%。结论 广西麻鸭可作为乙型肝炎病毒感染动物模型。实时荧光定量PCR法检测DHBV敏感性较高，重复性好，可用于DHBV检测。 |
| 关键词: 广西麻鸭 动物模型 鸭乙型肝炎病毒 实时荧光定量PCR |
| DOI：10.3969/j.issn.1674-3806.2012.04.03 |
| 分类号:R 512.62 |
| 基金项目:广西自然科学基金资助项目(编号：桂科基0731066) |
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| Experimental study of guangxi brown spotted duck as hepatitis B virus infection animal model |
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CHEN Zhao-ni, HUANG Ren-bin, LUO Xiu, et al.
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Department of Pharmacology, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the possibility of Guangxi brown spotted duck as hepatitis B virus infection animal model.Methods One-day old Guangxi brown spotted ducks were infected with duck hepatitis B virus (DHBV), and DHBV strong positive ducks were detected by the real-time fluorescence quantitative PCR (FQ-PCR) at 13 day after the infection of DHBV.Results One hundred and forty-eight serum samples in ducks were tested, and 136 samples (91.9%) were positive for DHBV.Conclusion The Guangxi brown spotted duck may be used as hepatitis B virus infection animal model. FQ-PCR method for detection of DHBV is sensitive, repeatable and practicable. |
| Key words: Guangxi brown spotted duck Animal model Duck hepatitis B virus Real-time fluorescence quantitative PCR |
