| 摘要: |
| ［摘要］ 目的 观察大鼠血清与舌组织IL-23表达水平的变化以及应用加味藿朴夏苓汤干预后的影响，探讨IL-23在湿热证发病中的作用和意义。方法 96只大鼠随机分为正常组、模型组和干预组。多因素复合造模方法制备温病湿热证大鼠模型，干预组第25天起予加味藿朴夏苓汤治疗，连服7 d。于造模后4、24、48、72 h四个时相点分批处死大鼠并取材。ELISA法检测大鼠血清IL-23的水平，SP免疫组化法检测舌组织IL-23的表达。结果 湿热模型组大鼠血清与舌IL-23表达水平在各时相点均显著高于正常组（P<0.01），药物干预组则在各时相点均显著低于模型组（P<0.01）。随时间点变化模型鼠血清与舌IL-23均呈逐渐下降趋势，在4 h时最高；血清IL-23的含量水平48 h和72 h时相点分别与4 h时相点比较差异有统计学意义（P<0.05），72 h时相点与24 h时相点比较差异亦有统计学意义（P<0.05）；舌IL-23的表达水平在各时相点分别比较差异均有统计学意义（P<0.01）。在湿热模型组病情分级为轻、中、重度的大鼠血清与舌IL-23的表达水平均呈逐渐升高趋势，组间比较差异均有统计学意义（P<0.01）；均与病情的严重程度呈正相关（P<0.01）。结论 IL-23参与了湿热证的发生、发展过程，其表达水平变化与湿热证的病情严重程度密切相关。加味藿朴夏苓汤可减少温病湿热证大鼠IL-23的表达，抑制炎症反应而减轻病情严重程度。 |
| 关键词: 湿热证 IL-23 加味藿朴夏苓汤 |
| DOI：10.3969/j.issn.1674-3806.2012.05.02 |
| 分类号:R 254.2 |
| 基金项目:广西自然科学基金资助项目（编号：2010GXNSFA013146）；广西卫生厅中医药科技专项课题（编号：GZKZ 10-111） |
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| The change of IL-23 in rat with syndrome of damp-heat and the intervention effect of modified huopuxialing decoction |
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LI Shu-guang,LU Jun-ying,QUE Tie-sheng,et al.
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Department of Traditional Chinese Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To observe the changes of levels of IL-23 in serum and tongue tissues and the intervention effect of modified huopuxialing decoction in order to study the effect and significance of IL-23 in the pathogenesis of damp-heat syndrome. Methods First,96 rats were randomly divided into normal group, damp-heat model group and medicine interference group.The damp-heat syndrome rat model was established by method of combining multi-factor. Medicine interference group was intervened by the modified huopuxialing decoction from the 25th day of modeling. Dosage was divided into two gavage and taken continuously for 7 days. At 4 h, 24 h, 48 h, 72 h after intervention, rats were sacrificed and sampled. The level of IL-23 in rat serum was detected by ELISA. The expression of IL-23 in rat tongue tissues was detected by SP-immunohistochemistry. Results The levels of expression of IL-23 in serum and tongue tissues of model group were significantly higher than those in the normal group in each time point（P<0.01）. The levels of expression of IL-23 in model group were significantly highter than those in medicine interferention group in each time point（P<0.01）. At 4h, the levels of IL-23 in serum were the highest in model group, and decreased gradually with time point and the levels of IL-23 at 72 h and 48 h were significant differences in comparison with the levels of IL-23 at 4 h（P<0.05）,between levels of IL-23 at 72 h and that at 24 h there was significant differences（P<0.05）. In IL-23 expression levels in tongue tissues, there were statistically significant difference between these time points（P<0.01）. In mild, moderate and severe rat model with syndrome of damp-heat, the levels of expression of IL-23 in serum and tongue tissues showed a gradual increasing trend with the clinical symptoms. There were significant difference between these groups (P<0.01) and the content of IL-23 in serum and tongue tissues were positively correlated to the severity of syndrome of damp-heat significantly（P<0.01）. Conclusion IL-23 maybe participated in the pathogenesis of syndrome of damp-heat and was significantly positively correlated to the severity of syndrome of damp-heat. Modified huopuxialing decoction can decrease the expression of IL-23 in rats with damp-heat syndrome, inhibit the inflammatory response and reduce the severity of the disease. |
| Key words: Syndrome of damp-heat IL-23 Modified huopuxialing decoction |
