| 摘要: |
| ［摘要］ 目的 探讨成熟T和NK细胞淋巴瘤与EB病毒感染的关系。方法 用原位杂交法检测121例成熟T和NK细胞淋巴瘤EBER（EB病毒编码的小RNA）表达情况，统计不同类型成熟T和NK细胞淋巴瘤与EB病毒感染相关性。结果 121例成熟T和NK细胞淋巴瘤EBER阳性率为63.6%(77/121)，其中结外者EBER阳性表达率为75.9%（60/79），阳性细胞多为肿瘤细胞，阳性细胞数>30%；而结内者EBER阳性表达率为40.5%(17/42)，阳性细胞多为母化的肿瘤细胞或反应性B淋巴细胞，阳性细胞数<10%。结外成熟T和NK细胞淋巴瘤EBER表达率显著高于结内（P<0.01）。EBER表达与组织学类型有关（P<0.01）。不同类型淋巴瘤EBER阳性表达率不同：结外鼻型NK/T细胞淋巴瘤（ENKCL）为98.0%（48/49），EBER阳性细胞数>30%；血管免疫母细胞性T细胞淋巴瘤（AITL）为77.8%（7/9），阳性细胞数<10%，阳性细胞多为反应性B细胞；外周T细胞淋巴瘤非特殊型（PT,NOS）为41.5%（17/41）；肠病相关性T细胞淋巴瘤（EATL）为33.3%（2/6）；间变性大细胞淋巴瘤并ALK阳性（ALCL，ALK+)者为18.2%（2/11）。结论 结外成熟T和NK细胞淋巴瘤发生与EB病毒感染密切相关，而结内成熟T和NK细胞淋巴瘤EB病毒感染可能为继发感染。检测肿瘤组织EBER表达有助于成熟T和NK细胞淋巴瘤的诊断和治疗。 |
| 关键词: 淋巴瘤 原位杂交法 EB病毒 |
| DOI：10.3969/j.issn.1674-3806.2012.08.17 |
| 分类号:R 733.7 |
| 基金项目: |
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| Investigation on the correlation between mature T and nature killer cell lymphomas with EBV infection |
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ZHOU Xiang-zhen, MO Xiang-lan,HUANG Zhen-lu,et al.
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Department of Pathology,the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To investigate the correlation between mature T and nature killer(NK) cell lymphomas with EBV infection.Methods The expression of EBER was detected using in situ hybridization method in 121 cases of mature T and nature killer cell lymphomas.Results The expression of EBER was seen in 63.6%(77/121) of the cases. The EBER positive cells were tumor cells, and more than 30% of the tumor cells were EBER expression in extranodal mature T cell and NK cell lymphomas, while in nodal mature T cell and NK cell lymphomas, the EBER positive cells were less than 10%, the positive cells were large transform tumor cells or reactive B cells. The EBER expression rate of extranodal mature T cell and NK cell lymphomas (75.9%,60/79) was higher than that of nodal mature T cell and NK cell lymphomas(40.5%,17/42)（P<0.01）.The positive rate of EBER was correlated with histopathological subtypes and location of diseases (P<0.01). 98.0%(48/49) of extranodal NK/T cell lymphoma(ENKCL) were EBV infection and the EBER positive cells were more than 30%, 77.8%(7/9) of angioimmunoblastic T cell lymphoma(AITL) were EBV infection, and the EBER expression cells were some of reactive B cells, the positive cells were less than 10%,while most of the tumor cells were negative to EBER. 41.5%(17/41) of peripheral T cell lymphoma non specificity(PT,NOS),33.3%(2/6) enteropathy-associated T-cell lymphoma(EATL) and 18.2%(2/11) of anaplastic large cell lymphoma with ALK positive ALCL(ALK+) were EBV infection.Conclusion EBV maybe play an important role on the development of extranodal mature T and nature killer cell lymphomas. Detecting the expression profile of EBER in mature T and nature killer cell lymphomas may be helpful to their diagnosis and therapy. |
| Key words: Mature T and nature killer cell lymphomas In situ hybridization EBER |
