| 摘要: |
| ［摘要］ 目的 为成功建立放射抗拒性鼻咽癌细胞系亚株，并探讨鼻咽癌放射抗拒机理。方法 采用类似临床放疗的照射模式反复照射从人鼻咽癌细胞系CNE-3中分离出具有放射抗拒性的新细胞系亚株。结果 采用类似临床放疗的照射模式反复照射分离出具有放射抗拒性的新细胞系亚株CNE-3R,，其在传代3个月后仍表现出较稳定的放射抗拒性，其SF2值均>0.5。结论 采用该研究方法能成功建立放射抗拒性鼻咽癌细胞系亚株，为研究鼻咽癌放射抗拒机理提供了必要的工具。 |
| 关键词: 反复照射 鼻咽癌 放射抗拒性 细胞系亚株 |
| DOI：10.3969/j.issn.1674-3806.2012.12.03 |
| 分类号:R 739.62 |
| 基金项目:广西自然科学基金资助项目（编号：桂科青0542041） |
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| Establishment of radioresistant subline from human nasopharyngeal carcinoma cell line by repeating irradiation |
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PENG Lu-xing, CHEN Jia-xin, CHENG Jin-jian, et al.
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Department of Radiatherapy, Center of Clinical Oncology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To establish radioresistant cell subline from a human nasopharyngeal carcinoma cell line CNE-3 and investigate the mechanism of radioresistance.Methods Radioresistant cell subline were established from a human nasopharyngeal carcinoma cell line CNE-3 by repeating X-ray irradiation as radiotherapy.The radiosensitivity of this subline,together with its parent CNE-3 cell line,were measured.Results This subline,named CNE-3R could be subcultured steadily three months,and kept SF2>0.5.Conclusion Radioresistant cell subline from a human nasopharyngeal carcinoma cell line successfully was established by using the method of research,which providing the tools for the study of the mechanism of radioresistance. |
| Key words: Repeating irradiation Nasopharyngeal carcinoma Radioresistance Cell subline |
