| 摘要: |
| ［摘要］ 目的 了解新诊断2型糖尿病外周血单个核细胞中氨基末端激酶（JNK）（Thr183/Tyr185）活性变化及其影响因素。方法 招募正常糖耐量健康志愿者60名（NGT组），新诊断2型糖尿病患者60例（NT2DM组）。应用western blot法检测外周血单个核细胞中JNK（Thr183/Tyr185）磷酸化水平。结果 NT2DM组JNK（Thr183/Tyr185）磷酸化水平显著高于NGT组，差异有统计学意义（P<0.01）。相关性分析显示，JNK（Thr183/Tyr185）磷酸化水平与体重指数（BMI）、空腹血糖（FBG）、低密度脂蛋白胆固醇（LDL-C）呈正相关（P<0.05），在剔除年龄、性别等相关影响因素之后，空腹血糖仍与JNK（Thr183/Tyr185）磷酸化水平独立相关。结论 JNK通路过度激活可能增加了患糖尿病的风险。 |
| 关键词: JNK 2型糖尿病 单个核细胞 胰岛素抵抗 相关性分析 |
| DOI：10.3969/j.issn.1674-3806.2013.01.02 |
| 分类号:R 587.1 |
| 基金项目:国家自然科学基金资助项目（编号：30860113）；广西卫生厅重点科研课题（编号:桂卫重200736） |
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| JNK activity in peripheral blood mononuclear cells of newly diagnosed type 2 diabetic patients and its influencing factors |
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LIU De-ying,LU Wen-sheng
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Department of Internal Medicine，the People’s Hospital of Lingyun County，Guangxi 533100，China
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| Abstract: |
| ［Abstract］ Objective To explore the change of JNK (Thr183/Tyr185) activity in the peripheral blood mononuclear cells in the newly diagnosed type 2 diabetes patients.Methods A total of 60 healthy volunteers with normal glucose tolerance (NGT group), 60 newly diagnosed type 2 diabetes patients (NT2DM group) were recruited in our study. JNK (Thr183/Tyr185) phosphorylation levels were delected in the peripheral blood mononuclear cells by western blot method.Results JNK (Thr183/Tyr185) phosphorylation levels in NT2DM group were significantly higher than that in NGT group(P<0.01). The correlation analysis showed that JNK (Thr183/Tyr185) phosphorylation levels were positively associated with body mass index, fasting blood glucose and low-density cholesterol (P<0.05). Under the situation of excluding the related factors such age, gender and so on, JNK (Thr183/Tyr185) phosphorylation levels were still independently associated with fasting blood glucose (P<0.05).Conclusion JNK pathway over-activation may increase the risk of diabetes. |
| Key words: JNK Type 2 diabetes Mononuclear cells Insulin resistance Relation analysis |
