引用本文:陈　琳，魏亚楠，李林茂，左文娟，黄定贵，张文欣，罗珍玉，杨　敏，覃素娇.基因转染CLD1对大肠癌细胞株SW480粘附作用的影响[J].中国临床新医学,2014,7(5):394-397.
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基因转染CLD1对大肠癌细胞株SW480粘附作用的影响
陈　琳，魏亚楠，李林茂，左文娟，黄定贵，张文欣，罗珍玉，杨　敏，覃素娇
530021　南宁，广西壮族自治区人民医院体检中心
摘要:
［摘要］　目的　探讨基因转染CLD1对大肠癌细胞株SW480粘附作用的影响。方法　对SW480CLD1(转染CLD1的SW480细胞株)、SW480control(转染了pEGFP-C1的SW480细胞株)、SW480细胞株分别进行细胞同质粘附实验和细胞异质粘附实验。结果　细胞同质粘附实验结果显示SW480CLD1细胞粘附细胞数量少于SW480control（P=0.000）和SW480细胞（P=0.000），SW480control的粘附细胞数与SW480无差异（P=0.376），粘附细胞数顺序为SW480CLD1control和SW480。异质粘附实验的结果显示SW480CLD1粘附细胞数多于SW480control(P=0.000)和SW480（P=0.000），差异有统计学意义。SW480control和SW480粘附细胞数无显著差异(P=0.334)。粘附细胞数顺序为SW480CLD1>SW480control和SW480。结论　CLD1基因转染后可减少SW480细胞之间的同质粘附作用，增加SW480细胞与Ⅳ型胶原的粘附性。
关键词:  大肠癌  CLD1（claudin-1）  基因转染  粘附实验
DOI：10.3969/j.issn.1674-3806.2014.05.03
分类号:R 730
基金项目:广西自然科学基金资助项目（编号：桂科自0832206）
The adhesive effects of colorectal cancer cells SW480 by CLD1 gene transfection
CHEN Lin, WEI Ya-nan, LI Lin-mao,et al.
Department of Medical Examination Center, the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China
Abstract:
［Abstract］　Objective　To investigate the adhesive effects of colorectal cancer cells SW480 by CLD1 gene transfection.Methods　Cell homotypic adhesive tests and cell-matrix adhesive tests were performed on SW480CLD1(SW480 by CLD1 gene transfection),SW480control(SW480 by pEGFP-C1 transfection) and SW480 cells.Results　The result of homotypic adhesive tests showed that the number of homotypic adhesive SW480CLD1 cells were less than those of SW480control（P=0.000）and SW480 cells（P=0.000）. No significant difference was detected between SW480control and SW480 cells(P=0.376). The sequence of number of adhesive cell were SW480CLD1control and SW480. The result of cell-matrix adhesive tests showed that the number of SW480CLD1 adhesive cells were more than those of SW480control(P=0.000) and SW480 cells(P=0.000), significant difference was detected. No significant difference was detected between SW480control and SW480 cells(P=0.334). The sequence of number of adhesive cell were SW480CLD1>SW480control and SW480.Conclusion　The homotypic adhesion was decrease between SW480 cells by CLD1 gene transfection.The adhesion was increase between SW480 cells and collagen Ⅳmediated by CLD1 gene transfection.
Key words:  Colorectal carcinoma  CLD1（claudin-1）  Gene transfection  Adhesive tests