| 摘要: |
| ［摘要］ 目的 了解临床分离脑膜败血伊丽莎白菌(EME)的耐药率并进行耐药表型分析。方法 采用微量肉汤稀释法（MIC）和KB法检测30株EME对22种抗菌药物的耐药率，用纸片法、改良三维试验法进行超广谱β-内酰胺酶（ESBLs）、头孢菌素酶（Ampc）和金属β-内酰胺酶(MBL)的表型确证试验。结果 30株EME对所有β-内酰胺类药物的耐药率达70%以上，而对环丙沙星和左氧氟沙星及含酶抑制剂的哌拉西林/他唑巴坦和头孢哌酮/舒巴坦有较好的敏感性。对万古霉素和利福平耐药率分别为23.3%和16.7%。所有菌株均检出ESBLs而未检出Ampc，18株（60.0%）细菌检出产MBL。结论 EME是多药耐药的细菌，产ESBLs和MBL，其中ESBLs对β-内酰胺类药物的耐药起主要作用。临床常用于治疗革兰阴性杆菌的亚胺培南和美罗培南对EME效果差，而用于治疗革兰阳性球菌的万古霉素和利福平对该菌有治疗活性，复方新诺明、米诺环素、含酶抑制剂类、氟喹喏酮类药物是治疗的首选。 |
| 关键词: 脑膜败血伊丽莎白菌 多药耐药 超广谱β-内酰胺酶 金属β-内酰胺酶 |
| DOI：10.3969/j.issn.1674-3806.2014.12.06 |
| 分类号:R 446 |
| 基金项目:广西卫生厅科研课题（编号：Z2013411） |
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| Analysis of drug resistance and resistance phenotypes of Elizabethkingia meningoseptica |
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CHEN Xing-chun, NONG Sheng-zhou, ZHAO Li, et al.
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Department of Clinical Laboratories, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate drug resistance and resistance phenotypes of Elizabethkingia meningoseptica（EME）.Methods The drug resistance rates of 30 strains to 22 antibiotics were detected by MIC and disk dilution method. Phenotypes of extended spectrum β-lactamase（ESBLs）, Ampc and metallo β-lactamase（MBL） were detected by disk diffusion and modified three dimension test.Results The drug resistance rates of 30 strains to β-lactam grop drugs were above 70%, all strains were more susceptible to trimethoprim-sulfa（SXT）, ciprofloxacin, levofloxacin, piperacillin/tazobactam, cefoperazone/sulbactam. The drug resistance rates to vancomycin and rifampi were 23.3% and 16.7% respectively. All the isolates were detected to have ESBLs but no Ampc, 18 strains（60.0%） had MBL.Conclusion EME was multi-drug resistance, it can produce ESBLs and MBL.While ESBLs was most responsible for the resistance to β-lactam group drugs. EME was more resistance to imipenem and meropenem which were used to cure most of the gram negative bacilli, but more susceptible to vancomycin and rifampin which were used to cure most of the gram positive coccus. SXT, minocycline,β-lactamase inhibitor and quinolones drugs may be the best choice for its infection. |
| Key words: Elizabethkingia meningoseptica（EME） Multi-drug resistance Extended spectrum β-lactamase（ESBLs） Metallo β-laetamase（MBL） |
