| 摘要: |
| ［摘要］ 目的 分析白细胞介素基因（IL）-1β-511和白细胞介素基因（IL）-10-592位点多态性与广西煤矿工人尘肺患者遗传易感性的关系。方法 对88例尘肺患者和44例粉尘接触者的职业史、尘肺病史等进行问卷调查；采集每个研究对象的外周静脉血，应用聚合酶链反应-限制性片断长度多态性（PCR-RFLP）技术检测白细胞介素基因IL-1β-511和IL-10-592位点多态性。结果 88例尘肺患者和44例粉尘接触者均为广西某矿务局工人，64例患者主要接触矽尘，诊断为矽肺；24例患者主要接触煤尘，诊断为煤工尘肺。电泳结果显示，矽肺组IL-1β-511基因野生型有13例，突变型有50例；IL-10-592基因野生型有35例，突变型有29例。煤工尘肺组IL-1β-511基因野生型有4例，突变型有20例；IL-10-592基因野生型有15例，突变型有9例。对照组IL-1β-511基因野生型有17例，突变型有27例；IL-10-592基因野生型和突变型各22例。矽肺组和煤工尘肺组IL-1β-511、IL-10-592等位基因频率与对照组比较，差异无统计学意义(P>0.05)。结论 白细胞介素基因IL-1β-511和IL-10-592位点多态性与广西煤矿工人尘肺的发病可能无关。 |
| 关键词: 尘肺 基因多态性 白细胞介素 |
| DOI：10.3969/j.issn.1674-3806.2015.04.01 |
| 分类号:R 135.2 |
| 基金项目:广西科学研究与技术开发计划项目（编号：桂科攻1140003B-83） |
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| Relationship between IL-1β-511 and IL-10-592 gene polymorphism and the susceptibility of pneumoconiosis in coal miners in Guangxi |
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MAI Zhi-dan, LI Xiao-ping, LIANG Mei, et al.
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Guangxi Zhuang Autonomous Region Institute for Prevention and Treatment of Occupational Diseases, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To analyze the relationship between gene interleukin(IL)-1β-511, interleukin gene(IL)-10-592 polymorphism and genetic susceptibility of pneumoconiosis in coal miners in Guangxi.Methods A questionnaire surrvey of occupational history, history of pneumoconiosis etc, was conducted among 88 pneumoconiosis patients and 44 dust-exposed workers. The peripheral venous blood was collected in each studied object, and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used to detect polymorphism of interleukin gene IL-1β-511 and IL-10-592.Results Eighty-eight pneumoconiosis patients and 44 dust-esposed workers were the workers in a Bureau of Mines in Guangxi. Among 88 pneumoconiosis patients, there were 64 patients with silicosis who were exposed primarily to silica dust; 24 patients with coal workers′ pneumoconiosis who were exposed primarily to coal dust. Electrophoresis showed that wild-type gene of IL-1β-511 in silicosis group was 13 cases, 50 cases of mutant and wild type gene of IL-10-592 was 35 cases, 29 cases of mutant; wild-type gene of IL-1β-511 in Coal workers′ pneumoconiosis group was 4 cases, 20 cases of mutant and wild type gene of IL-10-592 was 15 cases, 9 cases of mutant; wild-type gene of IL-1β-511 in the control group was 17 cases, 27 cases of mutant; wild type gene and mutant of IL-10-592 both were 22 cases. In comparison of IL-1β-511, IL-10-592 allele frequency in Coal workers′ pneumoconiosis group and silicosis group comparison with that in the control group, the difference was not statistically significant(P>0.05).Conclusion The interleukin gene IL-1β-511 and IL-10-592 gene polymorphism may be irrelevant to the incidence of Guangxi mine workers′ pneumoconiosis. |
| Key words: Pneumoconiosis Polymorphism Interleukin |
