| 摘要: |
| ［摘要］ 目的 探讨实时荧光定量PCR技术检测肺炎衣原体的诊断价值。方法 对呼吸道感染的186例患者的痰液分别采用实时荧光定量PCR技术和基因测序检测，以评价实时荧光定量PCR技术检测肺炎衣原体感染的准确性。结果 实时荧光定量PCR检测肺炎衣原体的灵敏度为100.0%，特异度为93.1%，阳性预期值为80.8%，阴性预期值为100.0%，准确率为94.6%。结论 实时荧光定量PCR技术检测肺炎衣原体具有较高的可靠性，适用于临床快速诊断肺炎衣原体感染的相关疾病。 |
| 关键词: 实时荧光定量PCR 肺炎衣原体 |
| DOI：10.3969/j.issn.1674-3806.2015.10.21 |
| 分类号:R 563.1 |
| 基金项目: |
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| Diagnosis of chlamydiales pneumoniae using real-time quantitative PCR |
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LUO Yan-fang, ZHAO Lin, LI You-qiong, et al.
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Department of Clinical Laboratory, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To study the diagnostic value of real-time quantitative PCR detection in chlamydiales pneumoniae.Methods Sputum samples from 186 patients with respiratory infection were detected using real-time quantitative PCR and gene sequencing respectively to evaluate the accuracy of real-time quantitative PCR for detecting chlamydiales pneumoniae.Results Taking gene sequencing as a gold standard, the sensitivity of real-time quantitative PCR was 100.0%; the specificity was 93.1%; the positive predictive value was 80.8%; the negative predictive value was 100.0%, and the accuracy was 94.6%.Conclusion Real-time quantitative PCR has high reliability and is suitable for rapid diagnosis of related diseases infected by chlamydiales pneumoniae. |
| Key words: Real-time quantitative PCR Chlamydiales pneumoniae |
