引用本文:高连丛,马莹慧,李平亚,韩 柳.新人参皂苷元HPD相关物质的分离鉴定研究[J].中国临床新医学,2019,12(5):506-509.
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新人参皂苷元HPD相关物质的分离鉴定研究
高连丛,马莹慧,李平亚,韩 柳
132013 吉林,吉林医药学院临床医学院(高连丛),药学院(马莹慧,韩 柳);130000 长春,吉林大学药学院(李平亚)
摘要:
[摘要] 目的 分离纯化新人参皂苷元羟基人参二醇(hydroxylation panoxadiol,HPD)的主要相关物质,并进行结构确证。方法 利用反复硅胶柱层析和重结晶方法对HPD合成过程中所产生的相关物质进行分离纯化,得到4个化合物,经理化性质及波谱学方法(LC-MS、1H-NMR、13C-NMR)鉴定其结构。结果 新人参皂苷元HPD中4个相关物质分别是(20S,24R)-达玛-20,24-环氧-3β,6α,12β,25-四醇、(20S,24S)-达玛-20,24-环氧-3β,12β,25-三醇、(20S,25R)-达玛-20,25-环氧-3β,12β,26-三醇和达玛-24-烯-3β,12β,20S-三醇。结论 新人参皂苷元HPD相关物质的定量和定性研究可以为HPD原料药的质量控制提供依据。
关键词:  新人参皂苷元HPD  相关物质  杂质  分离鉴定
DOI:10.3969/j.issn.1674-3806.2019.05.09
分类号:R 284
基金项目:吉林省科技发展计划项目(编号:20170520040JH)
Study on isolation and identification of related substances of new sapogenin hydroxylation panoxadiol
GAO Lian-cong, MA Ying-hui, LI Ping-ya, et al.
School of Clinical Medicine, Jilin Medical College, Jilin 132013, China
Abstract:
[Abstract] Objective To separate and purify the main related substances of new sapogenin hydroxylation panoxadiol(HPD), and to confirm their structures. Methods Four main impurities were isolated from new sapogenin HPD using repeated silica column chromatography and recrystallization. The structural characteristics of the impurities were determined by physical and chemical properties and spectral methods, such as LC-MS, 1H-NMR, and 13C-NMR. Results The structrues of the four related substances for new sapogenin HPD were identified as (20S, 24R)-dammar-20, 24-epoxy-3β, 6α, 12β, 25-tetraol, (20S, 24S)-dammar-20, 24-epoxy-3β, 12β, 25-triol, (20S, 25R)-dammar-20, 25-epoxy-3β, 12β, 26-triol and dammar-24-ene-3β, 12β, 20S-triol. Conclusion The quantitative and qualitative studies on the HPD related substances of new sapogenin HPD can provide a basis for the quality control of HPD active pharmaceutical ingredients.
Key words:  New sapogenin hydroxylation panoxadiol(HPD)  Related substances  Impurity  Isolation and identification