引用本文:孟 媛,许 斌.EMX2和β-catenin Cyclin D1在结直肠癌组织中的表达及临床意义[J].中国临床新医学,2019,12(9):984-988.
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EMX2和β-catenin Cyclin D1在结直肠癌组织中的表达及临床意义
孟 媛,许 斌
113006 辽宁,抚顺市中心医院病理科
摘要:
[摘要] 目的 观察空通气孔同源框2(EMX2)和β-连环蛋白(β-catenin)、细胞周期蛋白D1(Cyclin D1)在正常结直肠黏膜、结直肠癌组织中的表达情况,探讨结直肠癌发展过程中EMX2与β-catenin、Cyclin D1的关系。方法 S-P法检测EMX2和β-catenin、Cyclin D1在正常结直肠黏膜、结直肠癌组织中蛋白表达情况,并分析EMX2和β-catenin、Cyclin D1与结直肠癌临床病理特征之间及患者预后的关系。结果 (1)在正常结直肠黏膜、结直肠癌组织中EMX2阳性表达率分别为76.7%和38.2%(P<0.01),EMX2阳性表达率与结直肠癌临床分期及淋巴结转移有关。在正常结直肠黏膜、结直肠癌组织中β-catenin阳性表达率分别为6.7%、65.5%;Cyclin D1阳性表达率分别为10.0%、63.6%。β-catenin与Cyclin D1阳性表达率与结直肠癌的临床分期、肿瘤分化程度、淋巴结转移有关(P<0.05)。(2)在结直肠癌组织中EMX2和β-catenin表达呈负一致性(Kappa值=-0.389,P=0.000);EMX2与Cyclin D1表达一致性不明显(Kappa值=-0.161,P=0.054)。(3)应用Kaplan-Meier生存分析表明,结直肠癌组织中EMX2阳性表达与患者较好的预后有关(P=0.032)。结论 结直肠癌组织中EMX2阳性表达减少,EMX2低表达与结直肠癌患者的临床分期、淋巴结转移及预后密切相关。在结直肠癌患者中检测EMX2,可能成为判断患者临床分期和预后指标。
关键词:  空通气孔同源框2  β-连环蛋白  细胞周期蛋白D1  结直肠癌  免疫组织化学染色
DOI:10.3969/j.issn.1674-3806.2019.09.14
分类号:R 730
基金项目:
Expressions of EMX2, β-catenin and Cyclin D1 in colorectal cancer tissues and their clinical significances
MENG Yuan, XU Bin
Department of Pathology, Fushun Central Hospital, Liaoning 113006, China
Abstract:
[Abstract] Objective To observe the expressions of empty spiracles homeobox 2(EMX2),β-catenin and Cyclin D1 in normal colorectal mucosa and colorectal cancer tissues and to investigate the relationship between EMX2 and β-catenin, Cyclin D1 in the development of colorectal cancer. Methods The immunohistochemical S-P method was used to detect the expressions of EMX2, β-catenin and Cyclin D1 proteins in normal colorectal mucosa and colorectal cancer tissues. The correlations of EMX2,β-catenin and Cyclin D1 and clinicopathologic features in colorectal cancer and the patients′ prognosis were analyzed. Results (1)The positive rates of EMX2 expression was 76.7% and 38.2% in the normal colorectal mucosa and the colorectal cancer tissues, respectively(P<0.01). The positive rates of EMX2 expression in the colorectal cancer tissues were associated with the clinic stages and lymphatic metastasis. The positive expression rates of β-catenin in the normal colorectal mucosa and the colorectal cancer tissues were 6.7% and 65.5%, respectively. The positive expression rates of Cyclin D1 in the normal colorectal mucosa and the colorectal cancer tissues were 10.0% and 63.6%, respectively. The positive expression rates of β-catenin and Cyclin D1 were associated with the clinic stages, the degree of tumor differentiation and lymphatic metastasis(P<0.05). (2)EMX2 and β-catenin showed negative consistency in colorectal cancer tissues(Kappa=-0.389, P=0.000); The expressions of EMX2 and Cyclin D1 were not obvious(Kappa=-0.161, P=0.054). (3)Kaplan-Meier survival analysis showed that positive EMX2 expression in colorectal cancer tissues was associated with better prognosis(P=0.032). Conclusion Positive EMX2 expression reduces in colorectal cancer tissues, suggesting that the low expression of EMX2 is associated with clinic stages, lymphatic metastasis and prognosis. EMX2 detection in patients with colorectal cancer may be an indicator of clinical stage and prognosis.
Key words:  Empty spiracles homeobox 2(EMX2)  β-catenin  Cyclin D1  Colorectal cancer  Immunohistochemical staining