引用本文:潘春熹,吕立文.基于宏基因组的二代测序技术在重症肺炎患者病原体快速检测中的应用价值探讨[J].中国临床新医学,2020,13(4):370-373.
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基于宏基因组的二代测序技术在重症肺炎患者病原体快速检测中的应用价值探讨
潘春熹,吕立文
530021 南宁,广西壮族自治区人民医院急诊科
摘要:
[摘要] 目的 探讨基于宏基因组的二代测序技术(mNGS)在重症肺炎患者病原学检测中的应用价值。方法 纳入2018-07~2019-05收治的重症肺炎患者26例,比较mNGS与传统检测方法对病原菌的诊断效能,同时评价抗生素暴露对检出率的影响。结果 mNGS检出病原菌的阳性率为92.3%,高于传统培养方法的42.3%,差异有统计学意义(P<0.05)。在使用抗生素的前提下,mNGS的阳性率(91.3%)显著高于传统方法(43.5%),差异有统计学意义(P<0.05)。结论 mNGS对病原菌鉴定具有较高的灵敏度,而且使用抗生素对mNGS检测结果影响小,对感染患者的诊治及疗效有着重要的临床应用价值。
关键词:  宏基因组二代测序技术  诊断  感染
DOI:10.3969/j.issn.1674-3806.2020.04.12
分类号:R 446.1
基金项目:广西卫健委科研课题(编号:Z2016620)
Application value of metagenomic next-generation sequencing in rapid detection of pathogens in patients with severe pneumonia
PAN Chun-xi, LYU Li-wen
Department of Emergency, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
Abstract:
[Abstract] Objective To explore the application value of metagenomic next-generation sequencing(mNGS) in detection of pathogens in patients with severe pneumonia. Methods Twenty-six patients with severe pneumonia were enrolled from July 2018 to May 2019. The diagnostic efficacies of the mNGS and the traditional detection method on the pathogenic bacteria were compared, and the impact of antibiotic exposure on the detection rate was evaluated. Results The positive rate of pathogenic bacteria detected by mNGS(92.3%) was significantly higher than that detected by the traditional culture method(42.3%)(P<0.05). Under the premise of using antibiotics, the positive rate of pathogenic bacteria detected by mNGS(91.3%) was significantly higher than that detected by the traditional method(43.5%)(P<0.05). Conclusion mNGS has a high sensitivity to the identification of pathogenic bacteria, and the use of antibiotics has little effect on the detection results of mNGS, which has an important clinical application value for the diagnosis and treatment of infected patients.
Key words:  Metagenomic next-generation sequencing(mNGS)  Diagnosis  Infection