引用本文:林 鑫,刘 鹃,董 蓉,周朝敏,袁 静,查 艳.血清GM-CSF IL-18在急性肾损伤中的相关性及对急性肾损伤的预测作用[J].中国临床新医学,2020,13(9):878-881.
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血清GM-CSF IL-18在急性肾损伤中的相关性及对急性肾损伤的预测作用
林 鑫,刘 鹃,董 蓉,周朝敏,袁 静,查 艳
550002 贵阳,贵州省人民医院(国家卫生健康委员会肺脏免疫性疾病重点实验室)肾内科(林 鑫,董 蓉,周朝敏,袁 静,查 艳);550002 贵阳,贵州省中医药大学第一附属医院手术室(刘 鹃)
摘要:
[摘要] 目的 探讨血清粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白介素-18(IL-18)在急性肾损伤(AKI)中的相关性及对AKI的预测作用。方法 选取SPF级Wistar大鼠30只,采用随机数字表法将其分为假手术组(SHAM组,n=5)和AKI模型组(AKI组,n=25)。检测两组在0、24、48、72、96 h时间点的血清肌酐(SCr)、IL-18、GM-CSF的水平变化,分析其相关性。结果 在造模后,AKI组出现了肾小管间质细胞坏死及炎症细胞浸润情况,AKI组在0、24、48、72、96 h时间点的肾小管损伤评分呈先升高后降低的变化,在48 h时间点的肾小管损伤评分评分最高。AKI组24~96 h时间点的肾小管损伤评分均高于SHAM组,差异有统计学意义(P<0.05)。AKI组SCr水平随时间呈持续升高趋势;而IL-18、GM-CSF水平呈先上升后下降的变化。SHAM组SCr、IL-18、GM-CSF水平在不同时间点变化不显著。AKI组在造模后48、72、96 h的SCr水平显著高于SHAM组(P<0.05);AKI组在造模后48 h和72 h的IL-18、GM-CSF水平显著高于SHAM组(P<0.05)。Pearson相关分析结果显示,AKI组血清IL-18水平与SCr、GM-CSF呈正相关(P<0.05)。结论 在大鼠AKI模型中,血清GM-CSF与IL-18呈正相关,其峰值出现早于SCr,可作为预测AKI发生的标志物。
关键词:  粒细胞-巨噬细胞集落刺激因子  白介素-18  急性肾损伤  炎症
DOI:10.3969/j.issn.1674-3806.2020.09.07
分类号:R-332
基金项目:贵州省科技计划项目(编号:黔科合LH字【2016】7171,黔科合基础【2017】1102)
Correlation between serum concentrations of GM-CSF and IL-18 in acute kidney injury and their predictive roles in acute kidney injury
LIN Xin, LIU Juan, DONG Rong, et al.
Department of Nephrology, Guizhou Provincial People′s Hospital(Key Laboratory of Pulmonary Immunological Diseases of National Health Commission of China), Guiyang 550002, China
Abstract:
[Abstract] Objective To explore the correlation between serum concentrations of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-18(IL-18) in acute kidney injury(AKI)and their predictive roles in AKI. Methods Thirty specific pathogen free(SPF) Wistar rats were selected and divided into sham operation group(SHAM group, n=5) and AKI model group(AKI group, n=25) by random number table method. The varied levels of serum creatinine(SCr), IL-18 and GM-CSF were detected in the two groups at the time points of 0 h, 24 h, 48 h, 72 h and 96 h, and their correlation was analyzed. Results After modeling, renal tubulointerstitial cell necrosis and inflammatory cell infiltration occurred in the AKI group. The scores of renal tubular injury in the AKI group at the time points of 0 h, 24 h, 48 h, 72 h and 96 h were increased at first and then decreased. The scores of renal tubular injury at the time point of 48 h were the highest. The scores of renal tubular injury in the AKI group from 24 h to 96 h time points were higher than those in the SHAM group, and the differences were statistically significant(P<0.05). The levels of SCr in the AKI group showed a trend of continuous increase over time, while the levels of IL-18 and GM-CSF increased at first and then decreased. The levels of SCr, IL-18 and GM-CSF in the SHAM group did not change significantly at different time points. The levels of SCr in the AKI group were significantly higher than those in the SHAM group 48, 72 and 96 hours after modeling(P<0.05). The levels of IL-18 and GM-CSF in the AKI group were significantly higher than those in the SHAM group 48 and 72 hours after modeling(P<0.05). The results of Pearson correlation analysis showed that the level of serum IL-18 was positively correlated with SCr and GM-CSF in the AKI group(P<0.05). Conclusion In the rat AKI model, serum GM-CSF is positively correlated with IL-18, and its peak value appears earlier than that of SCr, which can be used as a marker to predict the occurrence of AKI.
Key words:  Granulocyte-macrophage colony stimulating factor(GM-CSF)  Interleukin-18(IL-18)  Acute kidney injury(AKI)  Inflammation