引用本文:陈舒影,余方友.呼吸道感染病原体检测技术与发展趋势[J].中国临床新医学,2022,15(10):894-899.
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呼吸道感染病原体检测技术与发展趋势
陈舒影,余方友
325000 浙江,温州医科大学附属第一医院检验科(陈舒影);200090 上海,同济大学附属上海市肺科医院检验科(余方友)
摘要:
[摘要] 由于传统呼吸道病原体检测手段的限制,导致临床诊断难、治疗用药难。呼吸道感染多联检产品时效性高,可及早识别感染,并指导抗感染药物的合理使用。各种便携式(快速)诊断技术产品发展迅速,临床应用前景广阔。宏基因组二代测序技术(mNGS)对呼吸道感染病原体的检测覆盖面广、信息量大,对呼吸道病原体的早期发现,特别是新发传染病的病原体快速诊断,起到非常重要的作用。基于规律间隔成簇短回文重复序列(CRISPR)的核酸检测技术、三代测序技术及基因芯片技术等快速检测新技术为呼吸道病原体的诊断开辟了新的方向。
关键词:  呼吸道病原体  宏基因组二代测序技术  规律间隔成簇短回文重复序列/规律间隔成簇短回文重复序列相关蛋白检测技术  三代测序技术  基因芯片
DOI:10.3969/j.issn.1674-3806.2022.10.02
分类号:R 56;R 446
基金项目:
Detection technologies of pathogens in respiratory tract infection and their development trend
CHEN Shu-ying, YU Fang-you
Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Zhejiang 325000, China
Abstract:
[Abstract] The limitations of traditional detection means of respiratory pathogens make clinical diagnosis and therapeutic medication difficult. The multiple joint detection products have high timeliness for respiratory tract infection. They can identify infections as soon as possible, and guide the rational use of anti-infective drugs. A variety of portable(rapid) diagnostic technology products are developing rapidly and have broad prospects for clinical application. The detection of pathogens in respiratory tract infection by metagenomics next-generation sequencing(mNGS) is extensive and informative, which plays a very important role in the early detection of respiratory pathogens, especially in the rapid diagnosis of a novel infectious disease. New rapid detection technologies such as clustered regularly interspaced short palindromic repeats(CRISPR)-based nucleic acid assay, third-generation sequencing technology and gene chip test have showed new detection directions for the diagnosis of respiratory pathogens.
Key words:  Respiratory pathogen  Metagenomics next-generation sequencing(mNGS)  Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein(CRISPR/Cas) assay  Third-generation sequencing technology  Gene chip