| 摘要: |
| ［摘要］ 目的 应用网络药理学探讨鸡血藤治疗再生障碍性贫血（AA）的潜在作用机制。方法 通过中药系统药理学数据库与分析平台(TCMSP)获取鸡血藤的活性成分及作用靶点基因，通过GeneCards数据库检索AA的相关靶点基因，通过韦恩图分析其交集。应用R软件对关键靶点进行GO功能富集分析和KEGG通路富集分析。应用Cytoscape 3.8.2软件构建药物-活性成分-关键靶点-通路-疾病网络。结果 通过TCMSP筛选获得24种活性成分，其对应的靶点基因56个。经GeneCards数据库检索，获得AA的疾病靶点基因1 665个。韦恩图分析结果显示其交集靶点基因21个。基于该21个关键靶点基因，GO功能富集分析共获得1 052个富集结果，包括生物过程（BP）1 012个、细胞组分（CC）13个、分子功能（MF）27个。KEGG通路富集分析共获得70条信号通路，主要通路包含细胞凋亡、p53信号通路、糖尿病并发症中的AGE-RAGE信号通路，以及病毒、癌症、传染病等疾病相关通路。结论 鸡血藤通过多成分、多靶点、多通路的相互作用发挥治疗AA的作用，为进一步探讨鸡血藤治疗AA的作用机制提供参考。 |
| 关键词: 鸡血藤 再生障碍性贫血 靶点基因 信号通路 网络药理学 |
| DOI：10.3969/j.issn.1674-3806.2022.11.14 |
| 分类号:R 966 |
| 基金项目:百色市科学研究与技术开发计划项目（编号：百科20184711） |
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| Exploring potential mechanisms of Spatholobus Suberectus Dunn in treatment of aplastic anemia based on network pharmacology |
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TAN Hong-di, LU Yi, WAN Yan-ni, et al.
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Department of Pharmacy, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, China
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| Abstract: |
| ［Abstract］ Objective To explore the potential mechanisms of Spatholobus Suberectus Dunn in treatment of aplastic anemia(AA) by using network pharmacology. Methods The active components and target genes of Spatholobus Suberectus Dunn were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), and the relevant target genes of AA were retrieved through the GeneCards database, and the Venn diagram was used to analyze their intersection. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on key targets by using R software. The drug-active ingredient-key target-pathway-disease network was constructed by using Cytoscape 3.8.2 software. Results Twenty-four active ingredients were obtained through TCMSP screening, and their 56 corresponding target genes were identified. One thousand six hundred and sixty-five disease target genes of AA were retrieved from the GeneCards database. The results of Venn diagram analysis showed that there were 21 intersecting target genes. Based on the 21 key target genes, a total of 1 052 enrichment results were obtained by GO functional enrichment analysis, including 1 012 biological processes(BP), 13 cellular components(CC), and 27 molecular functions(MF). A total of 70 signaling pathways were obtained by KEGG pathway enrichment analysis, and the main pathways included apoptosis, p53 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, and disease-related pathways such as viruses, cancer, and infectious diseases. Conclusion Spatholobus Suberectus Dunn plays a role in the treatment of AA through the interaction of multiple components, multiple targets and multiple pathways, which provides a reference for further exploring the mechanisms of Spatholobus Suberectus Dunn in the treatment of AA. |
| Key words: Spatholobus Suberectus Dunn Aplastic anemia(AA) Target gene Signaling pathway Network pharmacology |
