| 摘要: |
| ［摘要］ 目的 分析呼吸道合胞病毒核酸（RSV-RNA）检测阳性患儿的临床特征，为临床诊断和治疗儿童呼吸道合胞病毒感染（RSV）提供依据。方法 对深圳市盐田区人民医院2016-09～2017-09住院患儿中有呼吸道感染症状者进行咽拭子采样，提取RNA后用逆转录聚合酶链反应（RT-PCR）法检测RSV基因，测定病毒载量，同时抽血检测血常规和C反应蛋白(CRP)。结果 1 641例呼吸道感染患儿中RSV-RNA阳性114例,阳性率为6.95%。<6个月、6个月～<1岁、1岁～<2岁这三组阳性率分别为14.52%、10.18%、10.96%，显著高于3岁～<5岁组和≥5岁组，差异有统计学意义（P<0.05）；7～9月份组阳性率为15.01%，高于其他月份组，差异有统计学意义（P<0.05）；男童组阳性率为6.22%，女童组阳性率为7.94%，差异无统计学意义（P>0.05）；RSV-RNA阳性患者白细胞计数偏高占29.82%，白细胞计数正常占65.79%,白细胞计数偏低占4.39%；病毒载量在各年龄组、各性别组差异无统计学意义（P>0.05）；病毒载量与白细胞计数、中性粒细胞比例、淋巴细胞比例、CRP无显著相关性（P>0.05）。结论 RSV-RNA检测对小儿呼吸道感染诊断具有重要价值，血常规及CRP检测对于是否病毒感染所能提供信息有限。 |
| 关键词: 呼吸道合胞病毒 病毒载量 聚合酶链反应 |
| DOI：10.3969/j.issn.1674-3806.2018.08.07 |
| 分类号:R 446.9 |
| 基金项目:深圳市盐田科技局科技计划资助项目（编号：20160102） |
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| Analysis of positive cases of respiratory syncytial virus in hospitalized children |
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JIN Xian, WU Yi, FAN Chun-hui, et al.
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Department of Laboratory Medicine, the People′s Hospital of Yantian District, Shenzhen City, Guangdong 518081, China
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| Abstract: |
| ［Abstract］ Objective To analyze the clinical characteristics of positive cases of respiratory syncytial virus ribonucleic acid(RSV-RNA) in hospitalized children and to provide the basis for clinical diagnosis and treatment of respiratory syncytial virus(RSV) infection in children. Methods Oropharyngeal swabs were obtained from the children with respiratory infection symptoms in the People′s Hospital of Yantian District in Shenzhen City during September 2016 and September 2017. RNAs were extracted from the oropharyngeal swabs and the RSV gene was detected by RT-PCR method and the viral load was detected. At the same time, the blood was drawn to detect the routine blood test and C-reactive protein(CRP). Results There were 114 RSV-RNA positive cases in 1641 children with respiratory infection, and the positive rate was 6.95%. The positive rates of the three groups (<6 months, 6 months～<1 year, 1 year～<2 years) were 14.52%, 10.18% and 10.96%, respectively, which were higher than those of the two groups (3 years～<5 years and ≥5 years)(P<0.05). The positive rate during July and September was 15.01% which was significantly higher than those in the other months(P<0.05). The positive rates were 6.22% and 7.94% in the boys and girls respectively(P>0.05). The leucocyte count of the RSV-RNA positive patients on the high side was 29.82%, on the normal side 65.79%, and on the low side 4.39%. Viral load was not significantly different in each age groups and sex groups(P>0.05), and there was no significant correlation between viral load and leucocyte count, neutrophil ratio, lymphocyte ratio, and CRP(P>0.05). Conclusion RSV-RNA detection has important value in the diagnosis of children′s respiratory infection. Blood routine and CRP detection provide limited information for virus infection. |
| Key words: Respiratory syncytial virus Viral load Polymerase chain reaction |
