| 摘要: |
| ［摘要］ 目的 观察氯胺酮联合丙泊酚在食蟹猴动物实验中的麻醉效果。方法 选取24只4～7岁雄性食蟹猴，按随机数字表法分为小牛血清组、四氯化碳组、酒精组及对照组，每组6只，造模18周后采用氯胺酮基础麻醉+丙泊酚静脉麻醉对食蟹猴行枪式肝穿活检进行麻醉，术中监测生命体征和观察麻醉效果，并对其进行统计学分析。结果 造模18周后，3个造模组食蟹猴均出现肝纤维化，但以四氯化碳组肝纤维化明显；24例食蟹猴氯胺酮肌注起效时间为（4.20±1.55）min，各组氯胺酮肌注起效时间比较差异无统计学意义（P>0.05)；24例食蟹猴刺激睁眼时间为（6.50±2.84）min，各组刺激睁眼时间比较差异无统计学意义（P>0.05)；在氯胺酮联合丙泊酚麻醉实验中,四组心率、呼吸均平稳，肝穿前后呼吸末CO2分压、心率在组内比较及3个造模组与对照组比较差异均无统计学意义（P>0.05)；四组肝穿前后血糖均变化明显，组内肝穿前后比较差异有统计学意义（P<0.05)，但3个造模组与对照组比较差异均无统计学意义（P>0.05)。四组食蟹猴麻醉过程中均无无躁动、嚎叫表现，且苏醒迅速，术后无呕吐、抽搐等不良反应。结论 四氯化碳可有效地进行食蟹猴肝纤维化模型造模，氯胺酮基础麻醉+丙泊酚静脉麻醉在食蟹猴动物实验中应用麻醉效果良好，具有推广意义。 |
| 关键词: 食蟹猴 氯胺酮 丙泊酚 无痛麻醉 |
| DOI：10.3969/j.issn.1674-3806.2019.08.12 |
| 分类号:R 614.3 |
| 基金项目:广西科技基础条件平台建设资助项目（编号：1010821） |
|
| Effect of ketamine combined with propofol on anesthesia in animal experiment of cynomolgus monkeys |
|
LI Ping-ping, XIE Li-ping, WEI Zhu-mei, et al.
|
|
Department of Anesthesiology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
|
| Abstract: |
| ［Abstract］ Objective To observe the anesthetic effect of ketamine combined with propofol on cynomolgus monkeys. Methods Twenty-four male cynomolgus monkeys, aged 4～7 years, were selected and randomly divided into calf serum group, carbon tetrachloride group, alcohol group and control group, with 6 cases in each group. The monkeys were anesthetized with ketamine as basic anesthesia plus propofol intravenous anesthesia after 18 weeks of modeling and gunshot liver biopsy was performed on them. The vital signs were monitored during operation and the clinical anesthetic effect was observed, and statistical analysis was performed. Results Liver fibrosis occurred in all the three model groups after 18 weeks of modeling, but it was most obvious in the carbon tetrachloride group. The onset time of ketamine intramuscular injection in the 24 cynomolgus monkeys was (4.20±1.55)min, and there was no significant difference in the onset time among the groups(P>0.05). The stimulating eye opening time in the 24 cynomolgus monkeys was (6.50±2.84)min, and there was no significant difference in the stimulating eye opening time among the groups(P>0.05). The heart rate and breathing were steady in the four groups receiving ketamine combined with propofol for anesthesia, and there were no significant differences in the end-tidal carbon dioxide pressure and heart rate before and after liver puncture in intra-group comparison and in comparison of the three model groups with the control group(P>0.05). The changes of blood sugar were obvious before and after liver puncture in the four groups, and there were significant differences in the blood sugar levels before and after liver puncture in intra-group comparison(P<0.05). However, there were no significant differences in the blood sugar levels between the three model groups and the control group(P>0.05). There were no restlessness, howling, rapid recovery in the anesthesia process of the four groups, and no adverse reactions such as vomiting and convulsion after operation. Conclusion Carbon tetrachloride can be used to effectively establish the model of hepatic fibrosis in cynomolgus monkeys. Ketamine basic anesthesia plus propofol intravenous anesthesia has a good anesthetic effect on cynomolgus monkeys, which has a popularization significance. |
| Key words: Cynomolgus monkey Ketamine Propofol Painless anesthesia |
