| 摘要: |
| ［摘要］ 目的 探讨人脑胶质瘤中黑色素瘤相关抗原（MAGE）-D4和钙联蛋白（CANX） mRNA的表达水平，分析其相关性及临床意义。方法 应用基因表达谱交互式分析（GEPIA）数据库数据［胶质母细胞瘤（GBM）组织163例，低级别胶质瘤（LGG）组织518例，正常脑组织207例］分析MAGE-D4和CANX mRNA在人脑胶质瘤组织中的表达情况，并通过逆转录-定量聚合酶链反应（RT-qPCR）法在临床样本（胶质瘤62例，正常脑组织11例）中进一步验证。分析MAGE-D4和CANX mRNA表达水平与胶质瘤患者临床指标的关联性。应用小干扰RNA（siRNA）技术沉默胶质瘤细胞株SF126和U251中MAGE-D4的表达，探讨MAGE-D4与CANX表达的相关性。结果 GEPIA数据库数据分析结果显示，GBM和LGG组织的MAGE-D4和CANX mRNA表达水平均高于正常脑组织，差异有统计学意义（P<0.05）。针对临床样本的RT-qPCR检测结果也显示，胶质瘤组织中MAGE-D4和CANX mRNA的表达水平显著高于正常脑组织（P<0.05）。MAGE-D4及CANX mRNA的表达水平与患者年龄、性别、世界卫生组织（WHO）分级、病理类型、卡氏评分（KPS）、肿瘤最长径，以及Ki-67、胶质纤维酸性蛋白（GFAP）、S-100和波形蛋白（vimentin）的表达水平均无关联性（P>0.05）。Pearson相关分析结果显示，临床胶质瘤样本组织中MAGE-D4与CANX mRNA表达水平呈正相关（r=0.916，P=0.000）。细胞实验结果显示，经MAGE-D4 siRNA沉默后，SF126和U251细胞CANX mRNA的表达水平显著下降（P<0.05）。结论 人脑胶质瘤中MAGE-D4、CANX高表达，且二者表达水平呈正相关，提示其可能共同参与了胶质瘤的发生、发展，具有成为胶质瘤免疫治疗靶点的应用前景。 |
| 关键词: 胶质瘤 黑色素瘤相关抗原-D4 钙联蛋白 相关性 |
| DOI：10.3969/j.issn.1674-3806.2022.02.05 |
| 分类号:R 739.41 |
| 基金项目:国家自然科学基金项目（编号：81560408）；广西自然科学基金项目（编号：2018GXNSFAA050058，2018GXNSFAA281050）；广西区域性高发肿瘤早期防治研究教育部重点实验室课题（编号：GK2019-08，GKE-ZZ202006） |
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| Expressions of MAGE-D4 and CANX mRNA in human gliomas and their correlation analysis |
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XIE Huan, ZHANG Qing-mei, ZHANG Peng-bo, et al.
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Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the expression levels of melanoma-associated antigen(MAGE)-D4 and calnexin(CANX) mRNA in human gliomas, and to analyze their correlation and clinical significances. Methods The expressions of MAGE-D4 and CANX mRNA in human glioma tissues were analyzed using the data from Gene Expression Profiling Interactive Analysis(GEPIA) database［163 cases of glioblastoma(GBM) tissues, 518 cases of low-grade glioma(LGG) and 207 cases of normal brain tissues］, and were further verified in clinical samples(62 cases of glioma and 11 cases of normal brain tissues) by reverse transcription-quantitative polymerase chain reaction(RT-qPCR). The correlation between the expression levels of MAGE-D4 and CANX mRNA and the clinical indicators of glioma patients was analyzed. Small interfering RNA(siRNA) technology was used to silence the expressions of MAGE-D4 in glioma cell lines SF126 and U251, and the correlation between the expressions of MAGE-D4 and CANX was investigated. Results The results of data analysis of GEPIA database showed that the expression levels of MAGE-D4 and CANX mRNA in GBM and LGG tissues were higher than those in normal brain tissues, and the differences were statistically significant(P<0.05). The RT-qPCR test results of the clinical samples also showed that the expression levels of MAGE-D4 and CANX mRNA in glioma tissues were significantly higher than those in normal brain tissues(P<0.05). The expression levels of MAGE-D4 and CANX mRNA were not related to the patient′s age, gender, World Health Organization(WHO) grade, pathological type, Karnofsky Performance Score(KPS), the longest diameter of the tumor, and Ki-67, glial fibrillary acidic protein(GFAP), S-100 and vimentin expression levels(P>0.05). The results of Pearson correlation analysis showed that the expression levels of MAGE-D4 and CANX mRNA in the tissues of the clinical glioma samples were positively correlated(r=0.916, P=0.000). The cell experiment results showed that the expression levels of CANX mRNA in SF126 and U251 cells decreased significantly after they were silenced by MAGE-D4 siRNA(P<0.05). Conclusion MAGE-D4 and CANX are highly expressed in human gliomas, and their expression levels are positively correlated, suggesting that they may participate in the occurrence and development of gliomas, and have the application prospect of becoming targets for immunotherapy of gliomas. |
| Key words: Glioma Melanoma-associated antigen(MAGE)-D4 Calnexin(CANX) Correlation |
