引用本文:曹宇生,梁浩源,李林涛,蒋开海,黄天赋,李广生,黄许森.miR-3189-3p对结直肠癌患者预后的影响及作用机制探讨[J].中国临床新医学,2024,17(2):206-211.
【打印本页】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 535次   下载 500 本文二维码信息
码上扫一扫!
分享到: 微信 更多
miR-3189-3p对结直肠癌患者预后的影响及作用机制探讨
曹宇生1,梁浩源1,李林涛1,蒋开海1,黄天赋1,李广生1,黄许森2
1.右江民族医学院临床医学院,百色 533000;2.右江民族医学院附属医院胃肠外科,百色 533000
摘要:
[摘要] 目的 探讨miR-3189-3p对结直肠癌(CRC)患者预后的影响及作用机制。方法 基于CancerMIRNome数据库和ENCORI数据库数据分析miR-3189-3p在CRC组织和正常组织中的表达差异,探讨miR-3189-3p表达水平对患者生存预后的影响。分别将hsa-miR-3189-3p mimics、miR-3189-3p NC转染至LOVO细胞,通过Western blot实验探讨miR-3189-3p对LOVO细胞上皮细胞-间充质转化(EMT)关键蛋白[波形蛋白(vimentin)和N钙黏蛋白(N-cadherin)]表达水平的影响,通过划痕实验和Transwell实验探讨miR-3189-3p对LOVO细胞迁移和侵袭能力的影响。结果 CRC组织的miR-3189-3p表达水平显著高于正常组织(P<0.05)。miR-3189-3p高表达CRC患者的生存预后较低表达者更好(P<0.05)。过表达miR-3189-3p后,LOVO细胞的迁移和侵袭能力显著减弱(P<0.05),vimentin和N-cadherin蛋白表达水平显著降低(P<0.05)。结论 miR-3189-3p可能通过调控CRC细胞的迁移、侵袭以及EMT对患者的生存预后造成影响。
关键词:  miR-3189-3p  结直肠癌  上皮细胞-间充质转化  迁移  侵袭
DOI:10.3969/j.issn.1674-3806.2024.02.15
分类号:R 735.3
基金项目:
An exploration on the effect of miR-3189-3p on prognosis of patients with colorectal cancer and its mechanism of action
CAO Yusheng1, LIANG Haoyuan1, LI Lintao1, JIANG Kaihai1, HUANG Tianfu1, LI Guangsheng1, HUANG Xusen2
1.Clinical Medical School, Youjiang Medical University for Nationalities, Baise 533000, China; 2.Department of Gastrointestinal Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China
Abstract:
[Abstract] Objective To explore the effect of micro ribonucleic acid-3189-3p(miR-3189-3p) on prognosis of patients with colorectal cancer(CRC) and its mechanism of action. Methods Based on the data of CancerMIRNome database and ENCORI database, the differences in the expressions of miR-3189-3p in CRC tissues and normal tissues were analyzed, and the effect of the level of miR-3189-3p expression on the survival prognosis of the patients was explored. Hsa-miR-3189-3p mimics and miR-3189-3p NC were transfected into LOVO cells, respectively. The effects of miR-3189-3p on the expression levels of the key proteins(vimentin and N-cadherin) of epithelial-mesenchymal transition(EMT) in the LOVO cells were explored by using Western blot assay. The effects of miR-3189-3p on the migration and invasion ability of the LOVO cells were explored by using scratch assay and Transwell assay. Results The level of miR-3189-3p expression in the CRC tissues was significantly higher than that in the normal tissues(P<0.05). The survival prognosis of the CRC patients with high expression of miR-3189-3p was better than that of the CRC patients with low expression of miR-3189-3p(P<0.05). After overexpression of miR-3189-3p, the migration and invasion ability of the LOVO cells were significantly weakened(P<0.05), and the expression levels of vimentin and N-cadherin proteins were significantly decreased(P<0.05). Conclusion MiR-3189-3p may affect the survival prognosis of the patients by regulating the migration, invasion and EMT of CRC cells.
Key words:  Micro ribonucleic acid-3189-3p(miR-3189-3p)  Colorectal cancer(CRC)  Epithelial-mesenchymal transition(EMT)  Migration  Invasion