| 摘要: |
| 目的 本研究旨在探索具有良好生物相容性的多功能纳米材料FeS-PEG的载药性能,以及其在载带抗肿瘤小分子药物DOX后DOX对肿瘤细胞4T1细胞的增殖,入胞,凋亡的影响。方法 采用高温合成法合成纳米材料FeS,经层层交联修饰FeS得到FeS–PEG；分析FeS-PEG的细胞毒性、载药性能并进行载药入胞和细胞凋亡检测。结果 FeS–PEG纳米材料对抗肿瘤药物DOX的载药率为134%,载药后在PH=7.4的环境下释药7.13%,PH=6释药10.94%,PH=5释药24.53%。并且FeS–PEG载药后可将DOX药物滞留于细胞质中；游离DOX培养细胞促进细胞凋亡比例为(5.1±0.72)%,而FeS–PEG载药后可促进细胞凋亡比例(31.28±2.28)%。结论 FeS–PEG纳米材料可载带小分子药物,改变小分子药物在细胞内的停留部位,将小分子药物滞留于细胞质中,另外,该纳米材料载带药物后可明显促进细胞凋亡,从而达到增强药物对肿瘤细胞的持久杀伤作用,在药物载带输送方面具有很大应用前景。 |
| 关键词: 纳米材料 载药 药物入胞 细胞凋亡 |
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| The study for PEGylated FeS nanoparticle as carrier for drug delivery and the killing effect of tumor cells |
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shenweiqiang
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| Abstract: |
| In this work, we develop a simple PEGylated FeS nanoplates as a Nano-drugcarrier of anticancerdrug, with a saturated maximal DOX loading efficiency at ～134%.The obtained FeS-PEG nanoparticles exhibited great stability in physiological solutions. Notably, the FeS-PEG could carry the DOX into and stay the cytoplasm. In addition,the FeS-PEG carring DOX can promote tumor cell apoptosis obviously compared with the free DOX. Therefore, our work presented a new type of Nano-drugcarrier for treatment of cancer. |
| Key words: Nanoparticle Drugcarrier Tumor therapy Endocytosis |
