| 引用本文: | 刘珊,刘馨元,李亚陶,张靖熙,唐琴,王思毓,沈红梅.体外同期放化疗抵抗结直肠癌细胞模型的建立及其mRNA表达谱的变化[J].中国临床新医学,0,():-. |
| LIU Shan,LIU Xin-yuan,LI Ya-tao,ZHANG Jing-xi,TANG Qin,WANG Si-yu,SHEN Hong-mei.体外同期放化疗抵抗结直肠癌细胞模型的建立及其mRNA表达谱的变化[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 体外构建同期放化疗抵抗结直肠癌细胞模型,探讨其mRNA表达谱的变化。方法 模拟临床大剂量冲击疗法构建体外同期放化疗抵抗结直肠癌细胞模型,mRNA芯片检测同期放化疗抵抗结直肠癌细胞与其母细胞中mRNA 表达谱的差异,初步筛选与结直肠癌同期放化疗抵抗相关的mRNA。结果 成功构建出体外同期放化疗抵抗结直肠癌细胞模型,mRNA芯片共检测出2倍以上变化、并且差异有统计学意义(P < 0.05)的mRNA(即差异表达mRNA)共3832条。占所有mRNA的13.9%；2倍以上升高的共1847条；2倍以上降低的共1985条；10倍以上升高为共40条；10倍以上降低的共61条。结论 同期放化疗抵抗结直肠癌细胞与其母细胞相比,mRNA表达谱发生显著变化,提示结直肠癌同期放化疗抵抗的分子调节过程有差异性表达mRNA参与。 |
| 关键词: 结直肠癌 同期放化疗抵抗 模型 mRNA芯片 |
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| 基金项目:国家自然科学(81960557),云南省教育厅科学研究(2016ZDX061),云南省科技厅-昆明医科大学联合专项项目（2017FE468（-219）），云南省医疗卫生单位内设研究机构科研项目(2017NS182),云南省科技厅-昆明医科大学联合专项项目(2018FE001(-256)),云南省医疗卫生单位内设研究机构科研项目(2018NS0057)作者单位：昆明医科大学第三附属医院 中西医结合临床研究中心 邮编(650118) |
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| Establishment of chemoradioresistant colorectal cancer cell line and study on expression profile of mRNA in it |
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LIU Shan, LIU Xin-yuan, LI Ya-tao, ZHANG Jing-xi, TANG Qin, WANG Si-yu, SHEN Hong-mei
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Department of Integrated Traditional and Western Medicine Institute,The third Affiliated Hospital of Kunming Medical University,Kunming
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| Abstract: |
| Objective To establish chemoradioresistant colorectal cancer cell line and analysis the expression profile variation of mRNA in it. Methods SimulateSclinical high doses therapy to establish HCT116 chemoradioresistant colorectal cancer cell line in vitro. Use mRNA microarray technology to inspect the difference of mRNA expression profile between HCT116 chemoradioresistant cell line and its parental cell line, screen out the mRNA with differential expression．Results Compare with normal HCT116 cell, 3832 mRNA which have more than 2 times variation and significant difference(P < 0.05)by statistical analysis are regarded as mRNA with differential expression, accounting for 13.9% of all mRNA, while 1847 increase more than 2 times and 1895 reduce more than 2 times, 40 increase more than 10 times, and 61 reduce more than 10 times. Conclusion mRNA expression profile of HCT116 chemoradioresistant cell line changes significantly in comparison with its parental cell line. The different mRNAs may participate in the critical section of molecular regulatory mechanism in colorectal chemoradioresistance. |
| Key words: colorectal cancer chemoradioresistance model mRNA mRNA microarray |
