| 引用本文: | 陈星霖,王忠山,孙国文,陈盛,童昕,秦海燕.TGF-β信号影响牙源性黏液瘤来源的间充质干细胞的成骨分化[J].中国临床新医学,0,():-. |
| .TGF-β信号影响牙源性黏液瘤来源的间充质干细胞的成骨分化[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 探究转化生长因子β（TGF-β）信号通路对牙源性黏液瘤来源的间充质干细胞（OMMSC）成骨分化的影响。方法 对临床接诊疑似牙源性黏液瘤的2例患者进行锥形术CT（CBCT）检查及病理学检查以确诊。收集牙源性黏液瘤组织及颌骨骨髓，使用组织块法分离培养细胞，通过细胞增殖实验、流式检测、茜素红染色进行细胞鉴定。成骨培养7天后进行实时荧光定量聚合酶链反应（RT-qPCR）检测，分析TGF-β信号通路以及成骨相关基因在OMMSCs和颌骨骨髓来源间充质干细胞（JBMSCs）中的表达结果。添加小分子药物SB431542及TGF-β1进行成骨培养，使用茜素红染色检测体外成骨分化的体外结果。结果 通过影像学及组织学结果确诊患者为牙源性黏液瘤，分离培养的贴壁细胞具有梭形形态，表达间充质干细胞表面标记物，并具有成骨分化能力，符合间充质干细胞的定义。与JBMSCs相比，OMMSCs具有更高的自我更新能力。PCR结果显示，TGF-β／Smad信号相关基因在OMMSCSs中高表达，但成骨相关基因（OPN）表达降低。茜素红染色结果显示SB431542可明显促进OMMSCs体外成骨分化，TGF-β1对OMMSCs体外成骨分化能力具有抑制作用，结果具有统计学差异。结论 本研究成功分离并鉴定了来自牙源性黏液瘤的间充质干细胞，发现TGF-β信号相关基因在OMMSCs中高度表达，TGF-β信号传导的抑制可增强OMMSCs体外成骨效果，有望通过调控TGF-β信号通路改善牙源性黏液瘤的骨缺陷。 |
| 关键词: OMMSCs，JBMSCs，成骨，TGF-β信号通路，smad，SB431542，TGF-β1 |
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| 基金项目:江苏省重点专科项目(CZ0009) |
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| TGF- β signaling affects osteogenic differentiation of mesenchymal stem cells derived from Odontogenic Myxoma |
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Department of Dental Implantology, Nanjing Stomatological Hospital, Medical School of Nanjing University
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| Abstract: |
| Objective To investigate the effect of TGF-β signaling on osteogenic differentiation of mesenchymal stem cells from Odontogenic myxoma (OMMSCs). Methods CBCT examination and postoperative pathological examination were performed in two patients with suspected odontogenic myxoma. The tissues of odontogenic myxoma and bone marrow of jaw were collected and cultured. The cells were identified by cell proliferation assay, flow cytometry and alizarin red staining. After 7 days of osteogenic culture, RT-qPCR was performed to analyze the expression of TGF-β signaling pathway and osteogenic related genes in OMMSCs and JBMSCs. Small molecule drugs SB431542 and TGF-β1 were added in osteogenic culture, and alizarin red staining was used to detect the in vitro results of osteogenic differentiation. Results The patient was confirmed as odontogenic myxoma by imaging and histological results. The isolated and cultured adherent cells had spindle shape, expressed MSC surface markers, and had osteogenic differentiation ability, conforming to the definition of MSC. Compared with JBMSCs, OMMSCs showed higher self-renewal capacity ability. PCR results showed that TGF-β / Smad signal-related genes were highly expressed in OMMSCSs, but expression of osteogenesis related genes (OPN) was decreased. Alizarin red staining showed that SB431542 could significantly promote the in vitro osteogenic differentiation of OMMSCs, and TGF-β1 could inhibit the in vitro osteogenic differentiation of OMMSCs, with statistical difference. Conclusions In this study, mesenchymal stem cells from odontogenic myxoma were successfully isolated and identified. It was found that TGF-β signaling related genes were highly expressed in OMMSC, and inhibition of TGF-β signal could enhance the in vitro osteogenic ability of OMMSCs, which is expected to be achieved by regulating TGF- β Signal pathway improves bone defects in odontogenic myxoma. |
| Key words: OMMSCs, JBMSCs, Osteogenesis, TGF-β signaling, smad, SB431542, TGF-β1 |
