| 引用本文: | 曹雷,吴春炜,陈海龙,王若琳,何泽.桑枝总生物碱片对肥胖小鼠的保护作用及对肠道菌群的影响[J].中国临床新医学,0,():-. |
| caolei.桑枝总生物碱片对肥胖小鼠的保护作用及对肠道菌群的影响[J].中国临床新医学,0,():-. |
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| 桑枝总生物碱片对肥胖小鼠的保护作用及对肠道菌群的影响 |
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曹雷1, 吴春炜2, 陈海龙1, 王若琳1, 何泽2
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1.长春中医药大学;2.长春中医药大学附属医院
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| 摘要: |
| 目的 探讨桑枝总生物碱片对肥胖小鼠的保护作用及对肠道菌群的影响。方法 选择雄性无特定病原体（SPF）级C57BL/6小鼠通过12周高糖高脂饮食喂养诱导肥胖小鼠模型，将成模小鼠按体质量分层随机分为模型组（M组）、阳性对照组（PC组）、桑枝总生物碱片（SZ-A）低剂量组（SZ-L组）、SZ-A中剂量组(SZ-M组)、SZ-A高剂量组（SZ-H组），每组10只，另设10只正常小鼠为空白组（N组）。N组及M组予等体积蒸馏水灌胃，PC组予辛伐他汀混悬液（2.6 mg/kg）灌胃，SZ-L组、SZ-M组、SZ-H组分别以20 mg/kg、40 mg/kg、80 mg/kg剂量灌胃，各组灌胃12周。比较各组小鼠的Lee’s指数，体质量，脂肪湿重，生化指标水平，肠道菌群α及β多样性，肠道菌群相对丰度。结果 与M组比较，SZ-A各剂量组Lee’s指数、体质量、eWAT、iWAT、BAT、TC、TG水平显著降低（P＜0.05），SZ-H组eWAT、iWAT及BAT水平显著低于PC组及SZ-L组（P＜0.05），LDL-C水平显著低于M组（P＜0.05）。SZ-H组Ace指数较M组、PC组、及SZ-L组显著升高（P＜0.05），Chao指数较PC组及SZ-L组显著升高（P＜0.05），Shannon指数较M组、PC组、SZ-L组及SZ-M组显著升高（P＜0.05）。 Coverage结果显示，各组群落覆盖度均趋近于1；PCoA分析结果显示，N组、M组、PC组及SZ-A各剂量组分离度较好，N组与M组水平直线距离较远，且SZ-A各剂量组菌群结构逐渐趋向于N组；NMDS分析结果显示，stress值=0.131＜0.2。门水平上，与N组比较，M组厚壁菌门（Firmicutes）、热脱硫杆菌门（Desulfobacterota）及大肠杆菌门（Patescibacteria）相对丰度显著升高（P＜0.05），拟杆菌门（Bacteroldota）相对丰度显著降低（P＜0.05）；与M组比较，SZ-H组Firmicutes及Patescibacteria相对丰度显著降低（P＜0.05），Bacteroldota相对丰度显著升高（P＜0.05）。属水平上，与M组比较，SZ-H组鼠杆菌属（norank_f_Muribaculaceae）相对丰度显著升高（P＜0.05），unclassified_f_Lachnospiraceae、梭状芽孢杆菌属（norank_f_norank_o_Clostridia_UCG-014）及脱硫弧菌（Desulfovibrio）属相对丰度显著降低（P＜0.05）。结论 SZ-A可部分抵抗高脂饮食诱导的体质量增加，减少脂肪积累及白色脂肪组织扩张，改善脂代谢紊乱及高脂饮食所致肠道菌群结构紊乱，提高菌群物种多样性及部分物种相对丰度。 |
| 关键词: 桑枝总生物碱片 肥胖 肠道菌群 小鼠 脂肪组织 |
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| Protective Effects of Sangzhi Alkaloid Tablets on Obesity in Mice and Their Impact on Gut Microbiota |
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caolei
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Changchun University of Chinese Medicine
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| Abstract: |
| [Abstract] Objective To investigate the protective effects of morus alba alkaloid on obese mice and their impact on the intestinal microbiota. Methods SPF-grade C57BL/6 male mice were induced into an obese model through a 12-week high-sugar, high-fat diet. They were then stratified by body weight and randomly assigned to model (M), positive control (PC), low-dose Morus Branch Total Alkaloid tablets (SZ-L), medium-dose SZ-A (SZ-M), and high-dose SZ-A (SZ-H) groups, each containing 10 mice. An additional 10 normal mice were designated as the blank group (N group). The N and M groups received distilled water orally, the PC group received simvastatin suspension (2.6 mg/kg), and the SZ-L, SZ-M, and SZ-H groups were orally administered SZ-A at doses of 20 mg/kg, 40 mg/kg, and 80 mg/kg, respectively, for 12 weeks. Comparative analyses were conducted on Lee’s index, body weight, adipose tissue wet weight, biochemical indicators, intestinal microbiota α and β diversity, and relative abundance among the groups. Results Compared to the M group, all doses of SZ-A significantly reduced Lee’s index, body weight, eWAT, iWAT, BAT, TC, and TG levels (P < 0.05),. Additionally, in the SZ-H group, eWAT, iWAT, and BAT levels were significantly lower than in the PC and SZ-L groups (P < 0.05), and LDL-C levels were significantly lower than in the M group (P < 0.05). The Ace index in the SZ-H group was significantly higher than in the M, PC, and SZ-L groups (P < 0.05), while the Chao index was significantly higher than in the PC and SZ-L groups (P < 0.05), and the Shannon index was significantly higher than in the M, PC, SZ-L, and SZ-M groups (P < 0.05). Coverage results showed that the coverage of each group's community approached 1. PCoA analysis indicated good separation among the N, M, PC, and SZ-A groups, with a greater distance between the N and M groups, and the community structure of the SZ-A groups gradually trending towards that of the N group. NMDS analysis showed a stress value of 0.131 < 0.2. At the phylum level, compared to the N group, the relative abundance of Firmicutes, Desulfobacterota, and Patescibacteria was significantly higher (P < 0.05) in the M group, while Bacteroidota was significantly lower (P < 0.05). Compared to the M group, the SZ-H group exhibited significantly lower relative Firmicutes and Patescibacteria (P < 0.05), and significantly higher Bacteroidota (P < 0.05). At the genus level, compared to the M group, the SZ-H group showed significantly higher norank_f_Muribaculaceae (P < 0.05), while unclassified_f_Lachnospiraceae, norank_f_norank_o_Clostridia_UCG-014, and Desulfovibrio were significantly lower (P < 0.05). Conclusion SZ-A partially mitigates weight gain induced by a high-fat diet, reduces fat accumulation and expansion of white adipose tissue, improves lipid metabolism, regulates the disrupted intestinal microbiota structure induced by a high-fat diet, enhances microbial species diversity, and increases the relative abundance of specific microbial species. Further validation of the exact mechanism is still needed in the future. |
| Key words: Sangzhi Alkaloid Tablets obesity gut microbiota mice adipose tissue |
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