| 摘要: |
| ［摘要］ 目的 探讨天然产物Garcinone C对鼻咽癌细胞氧化损伤的影响及相关机制。方法 体外培养鼻咽癌细胞株HONE1和HK1，分别给予0 μmol/L、5 μmol/L、7.5 μmol/L的Garcinone C进行干预。通过酶标仪检测细胞中脂质过氧化产物丙二醛（MDA）的含量。利用流式细胞术结合BODIPY 581/591 C11探针染色检测细胞内脂质过氧化水平。利用免疫荧光实验检测DNA损伤标志物γ-H2A.X的核内表达情况。通过Western blot检测氧化损伤相关蛋白γ-H2A.X、RAD50、p53、KEAP1的表达水平。结果 经Garcinone C处理后，HONE1和HK1细胞内MDA含量和脂质过氧化水平显著升高（P<0.05），γ-H2A.X表达量显著增加（P<0.05），γ-H2A.X、p53蛋白表达显著上升（P<0.05），KEAP1、RAD50蛋白表达显著下降（P<0.05）。结论 Garcinone C可通过诱导鼻咽癌细胞氧化损伤而发挥抗肿瘤作用，有望成为治疗鼻咽癌的候选先导化合物。 |
| 关键词: Garcinone C 鼻咽癌 氧化损伤 |
| DOI：10.3969/j.issn.1674-3806.2026.04.06 |
| 分类号:R 739.6 |
| 基金项目:国家自然科学基金项目（编号：82260721）；广西医科大学研究生教育创新计划项目（编号：YCSW2025264） |
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| Study on effects of Garcinone C on oxidative damage in nasopharyngeal carcinoma cells and the related mechanisms |
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ZHAO Wenya1, BI Aozhen1, ZHAO Ming1, GUO Yulin1, HU Jingwen1, YIN Fuqiang2, LIU Xia1
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1.School of Basic Medical Sciences, Research Center for Translational Medicine, Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Guangxi Medical University, Nanning 530021, China; 2.Life Sciences Institute, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate effects of natural product Garcinone C on oxidative damage in nasopharyngeal carcinoma(NPC) cells and the related mechanisms. Methods NPC cell lines HONE1 and HK1 were cultured in vitro and were intervened with Garcinone C at concentrations of 0 μmol/L, 5 μmol/L and 7.5 μmol/L, respectively. The content of malondialdehyde(MDA), a lipid peroxidation product in cells, was detected by using a microplate reader. The levels of intracellular lipid peroxidation were detected by using flow cytometry combined with BODIPY 581/591 C11 probe staining. Immunofluorescence assay(IFA) was used to detect the expression of γ-H2A.X in the cell nucleus, a marker of DNA damage. Western blot was employed to detect the expression levels of oxidative damage-related proteins, including γ-H2A.X, RAD50, p53 and KEAP1. Results After the intervention with Garcinone C, the MDA content and lipid peroxidation levels in the HONE1 and HK1 cells were significantly increased(P<0.05), and the expression levels of γ-H2A.X were significantly increased(P<0.05), and the protein expressions of γ-H2A.X and p53 were significantly increased(P<0.05), and the protein expressions of KEAP1 and RAD50 were significantly decreased(P<0.05). Conclusion Garcinone C exerts anti-tumor effects via inducing oxidative damage in NPC cells and may serve as a promising lead compound for treatment of NPC. |
| Key words: Garcinone C Nasopharyngeal carcinoma(NPC) Oxidative damage |
