| 摘要: |
| ［摘要］ 目的 观察二苯乙烯苷（TSG）对老年性痴呆模型SAMP8鼠学习记忆能力和自由基清除的影响。方法 选用6月龄SAMP8鼠50只，随机均分为5组，每组10只：SAMP8对照组、阳性药石杉碱甲对照组、TSG低、中、高剂量组，另选用6月龄SAMR1鼠10只作正常对照组。各组分别灌胃相应药物50 d后，应用Morris水迷宫检测各组小白鼠学习记忆能力的变化和采用分光光度法测定各组小白鼠脑组织SOD、GSH-Px活性和MDA含量。结果 与SAMRl对照组比较，SAMP8鼠在隐蔽平台试验中表现出明显的学习记忆障碍，逃避潜伏期显著延长（P<0.05），TSG组从第3天起逃避潜伏期较SAMP8对照组显著缩短（P<0.05）；探索试验中二苯乙烯苷组原平台象限的停留时间和穿越原平台次数明显多于SAMP8对照组。SAMP8组脑组织SOD、GSH-Px活性明显降低，MDA含量明显升高，而TSG组能明显提高脑组织SOD、GSH-Px活性，降低MDA含量（P<0.05）。结论 二苯乙烯苷能改善SAMP8鼠的学习记忆能力及有效清除脑组织自由基。 |
| 关键词: 二苯乙烯苷 学习记忆能力 自由基 超氧化物歧化酶 谷胱甘肽过氧物酶 丙二醛 |
| DOI：10.3969/j.issn.1674-3806.2009.09.02 |
| 分类号:R 749.1 |
| 基金项目:广西高等学校优秀人才资助计划项目（编号：桂教人才0829） |
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| Effects of TSG on learning and memory abilities and free radical scavenging in SAMP8 mice |
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HUANG Ri-cai, HUANG Zhong-shi, LI Yun, et al.
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Department of Internal Neurology, People′s Hospital of Tianyang, Guangxi 533600, China
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| Abstract: |
| ［Abstract］ Objective To observe the effects of 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-D-glycoside (TSG) on learning and memory abilities and free radical scavenging in senescence accelerated-prone mouse/8 (SAMP8).Methods Fifty 6-month-old SAMP8 mice were randomized to 5 groups: SAMP8 group, huperzine A control group, low-, mid- and high-dose groups of TSG, with 10 mice in each group. Another 10 6-month-old SAMR1 mice were assigned to normal control group. After medication for 50 days, Morris water maze test was used to assess the change of learning and memory abilities and spectrophotometry was used to detect SOD and GSH-Px activity and MDA contents in each mouse brain.Results Compared with normal control group, SAMP8 mice showed significant impairments in learning and memory in hidden platform test, and the escape latency was significantly longer than that of TSG-treated group（P<0.05）. TSG-treated group could significantly increase the time and times of swim in original platform quadrant in spatial probe test（P<0.05）. SOD and GSH-Px activity decreased significantly and MDA contents increased significantly in SAMP8 brain. Compared with the SAMP8 group, TSG-treated groups could enhance SOD and GSH-Px activity and decrease MDA contents significantly in SAMP8 brain (P<0.05).Conclusion TSG could improve the learning and memory abilities and scavenge free radicals effectively in SAMP8 mice. |
| Key words: 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-D-glycoside (TSG) Learning and memory abilities Free radical Superoxide dismutase Glutathione peroxidase Malondialdehyde |
